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articles similar to
Stollberg et al. 10 (8): 1241
.
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106
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Method
:
Gene expression profiling of human breast tissue samples using SAGE-Seq
Zhenhua Jeremy Wu
,
Clifford A. Meyer
,
Sibgat Choudhury
,
Michail Shipitsin
,
Reo Maruyama
,
Marina Bessarabova
,
Tatiana Nikolskaya
,
Saraswati Sukumar
,
Armin Schwartzman
,
Jun S. Liu
,
Kornelia Polyak
,
and
X. Shirley Liu
Genome Res.
December 2010
20
:
1730
-
1739
;
Published in Advance
November 2, 2010
,
doi:
10.1101/gr.108217.110
...). Appropriate global diversitymeasurementswithin and across data sets are
evaluated
and used to cluster the libraries. We propose amapping strategy to align
SAGE
-Seq tags to the .We utilize themapping information tominimize sequencing errors andobtain accurate quantification of sense and antisense transcripts...
Abstract
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Methods
:
Large-scale production of SAGE libraries from microdissected tissues, flow-sorted cells, and cell lines
Jaswinder Khattra
,
Allen D. Delaney
,
Yongjun Zhao
,
Asim Siddiqui
,
Jennifer Asano
,
Helen McDonald
,
Pawan Pandoh
,
Noreen Dhalla
,
Anna-liisa Prabhu
,
Kevin Ma
,
Stephanie Lee
,
Adrian Ally
,
Angela Tam
,
Danne Sa
,
Sean Rogers
,
David Charest
,
Jeff Stott
,
Scott Zuyderduyn
,
Richard Varhol
,
Connie Eaves
,
Steven Jones
,
Robert Holt
,
Martin Hirst
,
Pamela A. Hoodless
,
and
Marco A. Marra
Genome Res.
January 2007
17
:
108
-
116
;
Published in Advance
November 29, 2006
,
doi:
10.1101/gr.5488207
...the usual practice of discarding such tags, we conclude that they should be retained to avoid introducing bias into the results and thereby maintain the
quantitative
nature of the data, which is a major theoretical advantage of
SAGE
as a tool for global transcriptional profiling. Footnotes ↵ 4...
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Resource
:
A quantitative atlas of polyadenylation in five mammals
Adnan Derti
,
Philip Garrett-Engele
,
Kenzie D. MacIsaac
,
Richard C. Stevens
,
Shreedharan Sriram
,
Ronghua Chen
,
Carol A. Rohl
,
Jason M. Johnson
,
and
Tomas Babak
Genome Res.
June 2012
22
:
1173
-
1183
;
Published in Advance
March 27, 2012
,
doi:
10.1101/gr.132563.111
...A
quantitative
atlas of polyadenylation in five mammals Adnan Derti 1 , 4 , Philip Garrett-Engele 2 , Kenzie D. MacIsaac 3 , Richard C. Stevens 2 , Shreedharan Sriram 1 , Ronghua Chen 1 , Carol A. Rohl 1 , Jason M...
Abstract
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LETTER
:
Quantitative evidence for conserved longevity pathways between divergent eukaryotic species
Erica D. Smith
,
Mitsuhiro Tsuchiya
,
Lindsay A. Fox
,
Nick Dang
,
Di Hu
,
Emily O. Kerr
,
Elijah D. Johnston
,
Bie N. Tchao
,
Diana N. Pak
,
K. Linnea Welton
,
Daniel E.L. Promislow
,
James H. Thomas
,
Matt Kaeberlein
,
and
Brian K. Kennedy
Genome Res.
April 2008
18
:
564
-
570
;
Published in Advance
March 13, 2008
,
doi:
10.1101/gr.074724.107
...
Quantitative
evidence for conserved longevity pathways between divergent eukaryotic species Erica D. Smith 1 , 2 , Mitsuhiro Tsuchiya 1 , Lindsay A. Fox 1 , Nick Dang 1 , Di Hu 1 , Emily O. Kerr 1 , Elijah D. Johnston 1...
Abstract
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Method
:
Mapping and quantifying nascent transcript start sites using TT-TSS-seq
Eleanor Elgood Hunt
,
Claudia Vivori
,
Richard Mitter
,
Vedis Agnadottir
,
and
Folkert J. van Werven
Genome Res.
March 2026
36
:
600
-
610
;
Published in Advance
February 17, 2026
,
doi:
10.1101/gr.280726.125
...transcripts but not of TSSs from unstable RNAs, such as long noncoding transcripts. Furthermore, using mature RNA prevents insights into transcription initiation dynamics. Therefore, nascent or newly transcribed RNA is required for detecting TSSs of unstable transcripts and
quantitative
analysis...
Abstract
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LETTER
:
Quantitative Assessment of Transcriptome Differences Between Brain Territories
Michel de Chaldée
,
Marie-Claude Gaillard
,
Nicolas Bizat
,
Jean-Marie Buhler
,
Olivier Manzoni
,
Joël Bockaert
,
Philippe Hantraye
,
Emmanuel Brouillet
,
and
Jean-Marc Elalouf
Genome Res.
July 2003
13
:
1646
-
1653
;
doi:
10.1101/gr.1173403
...a microassay for serial analysis of gene expression (
SAGE
) to generate
quantitative
mRNA expression profiles of normal adult mouse striatum, nucleus accumbens, and somatosensory cortex. Comparison of these profiles revealed 135 transcripts heterogeneously distributed in the brain. Among them, a majority (78...
Abstract
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Research
:
A national long-read sequencing study on chromosomal rearrangements uncovers hidden complexities
Jesper Eisfeldt
,
Adam Ameur
,
Felix Lenner
,
Esmee Ten Berk de Boer
,
Marlene Ek
,
Josephine Wincent
,
Raquel Vaz
,
Jesper Ottosson
,
Tord Jonson
,
Sofie Ivarsson
,
Sofia Thunström
,
Alexandra Topa
,
Simon Stenberg
,
Anna Rohlin
,
Anna Sandestig
,
Margareta Nordling
,
Pia Palmebäck
,
Magnus Burstedt
,
Frida Nordin
,
Eva-Lena Stattin
,
Maria Sobol
,
Panagiotis Baliakas
,
Marie-Louise Bondeson
,
Ida Höijer
,
Kristine Bilgrav Saether
,
Lovisa Lovmar
,
Hans Ehrencrona
,
Malin Melin
,
Lars Feuk
,
and
Anna Lindstrand
Genome Res.
November 2024
34
:
1774
-
1784
;
Published in Advance
October 29, 2024
,
doi:
10.1101/gr.279510.124
...using Megaruptor 3 (Diagenode). PacBio SMRTbell library construction was performed using the SMRTbell Template prep kit 3.0. SMRTbell libraries were size-selected either using AMPure beads or by the gel-based systems pippinHT (
Sage
Science) or
Sage
Elf (
Sage
Science). The library preparation procedure...
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LETTER
:
Controlled exchange of chromosomal arms reveals principles driving telomere interactions in yeast
Heiko Schober
,
Véronique Kalck
,
Miguel A. Vega-Palas
,
Griet Van Houwe
,
Daniel Sage
,
Michael Unser
,
Marc R. Gartenberg
,
and
Susan M. Gasser
Genome Res.
February 2008
18
:
261
-
271
;
Published in Advance
December 20, 2007
,
doi:
10.1101/gr.6687808
...Controlled exchange of chromosomal arms reveals principles driving telomere interactions in yeast Heiko Schober 1 , 2 , Véronique Kalck 1 , Miguel A. Vega-Palas 3 , Griet Van Houwe 2 , Daniel
Sage
4 , Michael Unser 4 , Marc R...
Abstract
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Method
:
Factors impacting target-enriched long-read sequencing of resistomes and mobilomes
Ilya B. Slizovskiy
,
Nathalie Bonin
,
Jonathan E. Bravo
,
Peter M. Ferm
,
Jacob Singer
,
Christina Boucher
,
and
Noelle R. Noyes
Genome Res.
November 2024
34
:
2048
-
2060
;
Published in Advance
November 5, 2024
,
doi:
10.1101/gr.279226.124
...that the differences in beta-diversity observed by enrichment protocol were associated primarily with differences between TELSeq and nonenriched libraries, with smaller differences between XT and XT-HS2 libraries.Another way to
evaluate
composition is through a binary heatmap (Fig. 5). This analysis revealed...
Abstract
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Resource
:
MicroRNA programs in normal and aberrant stem and progenitor cells
Christopher P. Arnold
,
Ruoying Tan
,
Baiyu Zhou
,
Si-Biao Yue
,
Steven Schaffert
,
Joseph R. Biggs
,
Regis Doyonnas
,
Miao-Chia Lo
,
John M. Perry
,
Valérie M. Renault
,
Alessandra Sacco
,
Tim Somervaille
,
Patrick Viatour
,
Anne Brunet
,
Michael L. Cleary
,
Linheng Li
,
Julien Sage
,
Dong-Er Zhang
,
Helen M. Blau
,
Caifu Chen
,
and
Chang-Zheng Chen
Genome Res.
May 2011
21
:
798
-
810
;
Published in Advance
March 30, 2011
,
doi:
10.1101/gr.111385.110
..., Miao-Chia Lo 5 , John M. Perry 6 , Valérie M. Renault 7 , Alessandra Sacco 1 , 2 , 8 , Tim Somervaille 9 , Patrick Viatour 10 , Anne Brunet 7 , Michael L. Cleary 9 , Linheng Li 6 , 11 , Julien
Sage
7 , 10...
Abstract
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