Searching journal content for articles similar to Steffens et al. 5 (4): 393.

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  1. PERSPECTIVE: Emerging technologies in DNA sequencing
    • Michael L. Metzker
    Genome Res. December 2005 15: 1767-1776; doi:10.1101/gr.3770505
    ...of a complementary Cy5-labeled-UTP or Cy5-labeled-dCTP substrate resulted in the spFRET signal. Following photobleaching of the Cy5 dye, the natural nucleotides dATP and dGTP were added to increase the nucleotide distance between subsequent Cy5-labeled dNTP additions, which would otherwise have resulted...
  2. LETTER: Direct linkage of mitochondrial genome variation to risk factors for type 2 diabetes in conplastic strains
    • Michal Pravenec,
    • Masaya Hyakukoku,
    • Josef Houstek,
    • Vaclav Zidek,
    • Vladimir Landa,
    • Petr Mlejnek,
    • Ivan Miksik,
    • Kristyna Dudová-Mothejzikova,
    • Petr Pecina,
    • Marek Vrbacký,
    • Zdenek Drahota,
    • Alena Vojtiskova,
    • Tomas Mracek,
    • Ludmila Kazdova,
    • Olena Oliyarnyk,
    • Jiaming Wang,
    • Christopher Ho,
    • Nathan Qi,
    • Ken Sugimoto,
    • and Theodore Kurtz
    Genome Res. September 2007 17: 1319-1326; Published in Advance August 10, 2007, doi:10.1101/gr.6548207
    ...by real-time PCR testing using QuantiTect SYBR Green reagents (QIAGEN) on an Opticon continuous fluorescence detector (MJ Research) with an annealing temperature of 58°C. Mitochondrial DNA levels were normalized relative to the internal genomic DNA control with relative amounts of mtDNA to genomic DNA...
  3. LETTER: A High-throughput AFLP-based Method for Constructing Integrated Genetic and Physical Maps: Progress Toward a Sorghum Genome Map
    • Patricia E. Klein,
    • Robert R. Klein,
    • Samuel W. Cartinhour,
    • Paul E. Ulanch,
    • Jianmin Dong,
    • Jacque A. Obert,
    • Daryl T. Morishige,
    • Shannon D. Schlueter,
    • Kevin L. Childs,
    • Melissa Ale,
    • and John E. Mullet
    Genome Res. June 1, 2000 10: 789-807; doi:10.1101/gr.10.6.789
    ...) and side pools (SP) were analyzed for the presence of two SSRs, Xtxp84 and Xtxp211 . BAC DNA pools positive for a given SSR are identified above the respective lane. Plant genomic DNA (BTx623) was used as PCR template in control lanes labeled BTx. Fluorescent-labeled molecular weight standards (LI...
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