Searching journal content for articles similar to Siegel et al. 14 (6): 1119.

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  1. ARTICLE: Rapid and reliable cloning of PCR products.
    • J B Lorens
    Genome Res. November 1991 1: 140-141; doi:10.1101/gr.1.2.140
    ...-specific oligonucleotide primer. Proc. Natl. Acad. Sci. 85 : 8998 – 9002 . 5. Lorens, J.B., unpublished. Sambrook, J., E.F. Fritsch, and T. Maniatis. 1989. Molecular cloning: A laboratory manual . Cold Spring Harbor Laboratory, New York. 7. Wilson, V.G. Wilson, V.G. 1988 . A rapid procedure for removal of excess linkers...
  2. Method: Restoring the potency of neutralizing antibody via guided hypermutation with hyper-antibody editor
    • Yuhong Wang,
    • Yuefeng Guo,
    • Qiuyu Lu,
    • Xinyi Liu,
    • Haoqi Xu,
    • Jiayi Chen,
    • Ruiqi Pi,
    • Shaopeng Yuan,
    • Ziting Yang,
    • Rusen Lu,
    • Fei-Long Meng,
    • Tingting Gan,
    • and Jiazhi Hu
    Genome Res. March 19, 2026 gr.281396.125; Published in Advance March 19, 2026, doi:10.1101/gr.281396.125
    ...co-directional LoxP sites, enabling 143 cleavage by Cre recombinase (Figure 2B). We tested five different introns to accommodate the 144 upstream LoxP site, including an artificial intron (Guzzardo et al. 2017), chimeric intron from CAG 145 8 promotor, a mutated form of the chimeric intron (mut...
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  3. Review: Advancements in prospective single-cell lineage barcoding and their applications in research
    • Xiaoli Zhang,
    • Yirui Huang,
    • Yajing Yang,
    • Qi-En Wang,
    • and Lang Li
    Genome Res. December 2024 34: 2147-2162; Published in Advance November 21, 2024, doi:10.1101/gr.278944.124
    ...the control of cell-type-specific promotors; therefore, its expression is confined into a group of cells instead of a single cell (Chen et al. 2022). This strategy often leads to sparsely distributed clones across a sample, making it challenging to distinguish clones from one another (Kester and van...
    OPEN ACCESS ARTICLE
  4. Method: Generation of mouse conditional knockout alleles in one step using the i-GONAD method
    • Renjie Shang,
    • Haifeng Zhang,
    • and Pengpeng Bi
    Genome Res. January 2021 31: 121-130; Published in Advance December 16, 2020, doi:10.1101/gr.265439.120
    ...(Supplemental Fig. S4G). Successful transmissions of the two loxP sites were also confirmed by PCR (Supplemental Fig. S4H) and sequencing (Fig. 2E, boxed panels).A cloning-based strategy to rapidly identify founders with loxP insertions in cisBy Mendel's law of inheritance, the simultaneous transmission of 5...
  5. REVIEW: Many Paths to Many Clones: A Comparative Look at High-Throughput Cloning Methods
    • Gerald Marsischky and
    • Joshua LaBaer
    Genome Res. October 15, 2004 14: 2020-2028; doi:10.1101/gr.2528804
    ...vectors, is based on the Cre- loxP -based site-specific recombination system of bacteriophage P1 ( Sternberg et al. 1981 ). Both Clontech systems are well-suited to automated methods. Creating Master Clones With In-Fusion Like the Gateway system, Clontech master (Donor) clones can be assembled using...
  6. METHODS: DNA Cloning Using In Vitro Site-Specific Recombination
    • James L. Hartley,
    • Gary F. Temple,
    • and Michael A. Brasch
    Genome Res. November 1, 2000 10: 1788-1795; doi:10.1101/gr.143000
    ...containing recombination sites and incubated with bacteriophage λ integrase recombination proteins to accomplish the transfer of the gene into the new vector. We refer to this process as recombinational cloning (RC). Our experiments initially used both the bacteriophage λ system and the Cre/ lox P system...
  7. LETTER: Systematic Cloning of Treponema pallidum Open Reading Frames for Protein Expression and Antigen Discovery
    • Matthew McKevitt,
    • Krupa Patel,
    • David Smajs,
    • Michael Marsh,
    • Melanie McLoughlin,
    • Steven J. Norris,
    • George M. Weinstock,
    • and Timothy Palzkill
    Genome Res. July 2003 13: 1665-1674; Published in Advance June 12, 2003, doi:10.1101/gr.288103
    ...is used for the initial cloning of PCR products, whereas the pHOST plasmid contains the appropriate promoter or tag sequences for creating fusion proteins. The recombinant protein expression construct is made by fusion of the pUNI and pHOST plasmids via Cre- loxP site–specific recombination ( Fig. 1 ; Liu...
  8. Research: Fusion, fission, and scrambling of the bilaterian genome in Bryozoa
    • Thomas D. Lewin,
    • Isabel Jiah-Yih Liao,
    • Mu-En Chen,
    • John D.D. Bishop,
    • Peter W.H. Holland,
    • and Yi-Jyun Luo
    Genome Res. January 2025 35: 78-92; Published in Advance January 6, 2025, doi:10.1101/gr.279636.124
    ...extensive scrambling has impacted Hox cluster organization in bryozoans. We annotated the Hox genes of the five bryozoan species (Supplemental Fig. S12; Supplemental Data S2) and identified extensive gene loss in the bryozoan Hox gene set, which lacks Hox1, Hox3, Scr, Antp, Lox2, and Post1 in all five...
    OPEN ACCESS ARTICLE
  9. Methods: Concerted Assembly and Cloning of Multiple DNA Segments Using In Vitro Site-Specific Recombination: Functional Analysis of Multi-Segment Expression Clones
    • David L. Cheo,
    • Steven A. Titus,
    • Devon R.N. Byrd,
    • James L. Hartley,
    • Gary F. Temple,
    • and Michael A. Brasch
    Genome Res. October 15, 2004 14: 2111-2120; doi:10.1101/gr.2512204
    ...Concerted Assembly and Cloning of Multiple DNA Segments Using In Vitro Site-Specific Recombination: Functional Analysis of Multi-Segment Expression Clones David L. Cheo 1 , 2 , Steven A. Titus 1 , 2 , Devon R.N. Byrd 2 , 3 , James L. Hartley 2 , 4...
  10. INSIGHT/OUTLOOK: QTL Mapping to QTL Cloning: Mice to the Rescue
    • Michel Georges
    Genome Res. July 1, 1997 7: 663-665; doi:10.1101/gr.7.7.663
    ...the achievable mapping resolution of QTL, posing a serious threat to the efficacy of positional (candidate) cloning for QTL considerably. QTL mapping efforts, whether performed in pedigrees or by exploiting linkage disequilibrium, are likely to leave geneticists with a portion of the that contains tens...
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