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Research: Loss of histone H3.3 results in DNA replication defects and altered origin dynamics in C. elegans
- Maude Strobino,
- Joanna M. Wenda,
- Laura Padayachy,
- and Florian A. Steiner
Genome Res. December 2020 30: 1740-1751; Published in Advance November 10, 2020, doi:10.1101/gr.260794.120
...to determine replication timing, map replication origins, and examine replication fork progression. Our analysis of the spatiotemporal regulation of DNA replication shows that despite the very rapid embryonic cell cycle, the is replicated from early and late firing origins and is partitioned into domains...
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LETTER: New genes with roles in the C. elegans embryo revealed using RNAi of ovary-enriched ORFeome clones
- Anita G. Fernandez,
- Kristin C. Gunsalus,
- Jerry Huang,
- Ling-Shiang Chuang,
- Nancy Ying,
- Hsiao-lan Liang,
- Caroline Tang,
- Aaron J. Schetter,
- Charles Zegar,
- Jean-François Rual,
- David E. Hill,
- Valerie Reinke,
- Marc Vidal,
- and Fabio Piano
Genome Res. February 2005 15: 250-259; doi:10.1101/gr.3194805
..., and scoring criteria (for review, see Piano and Gunsalus 2002 ). Different studies have varied in their use of dsRNAs derived from amplicons that include intronic sequences ( Gönczy et al. 2000 ; Piano et al. 2002 ; Kamath et al. 2003 ; Simmer et al. 2003 ) or from cDNA clones composed exclusively of exonic...

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