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  1. ...-linked chromatin from each set of five livers was pooled and used as starting material for immunoprecipitations with antibodies directed against POLR3A (RPC1) and POLR3D (RPC4), two RNAP-III subunits, POLR2B (RPB2), the second largest RNAP-II subunit, as well as histone H3 trimethylated on lysine 4 (H3K4me3...
  2. .... 1982 ). PCR analysis revealed that about half of the 10 tested VR sequences are present in genomic DNA from three other T. brucei strains ( Fig. 4A ). To test whether VR s have different expression control from that of VSG s, which yield mRNA in bloodstream stage trypanosomes in a clone...
  3. ...that overlap with tRNA and/or snRNA genes and also at the single TTR6 (sTTR6), which lacks such genes (Fig. 5A,B). We detected, consistent with T. brucei snRNA and tRNA genes being transcribed by RNAPIII (Nakaar et al. 1997; Tschudi and Ullu 2002), enrichment of RPC1 (the largest RNAPIII subunit...
  4. ...the two subunits, and the connection domain in the p51 subunit makes close contact with the tRNA primer annealed to the viral RNA template ( Kohlstaedt et al. 1992 ). Finally, the connection domain may even play a role in the incorporation of protein in the virus particle. Mutations in the connection...
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