Searching journal content for articles similar to Schuler 7 (5): 541.

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  1. Method: Quantitative mitochondrial DNA copy number determination using droplet digital PCR with single-cell resolution
    • Ryan O'Hara,
    • Enzo Tedone,
    • Andrew Ludlow,
    • Ejun Huang,
    • Beatrice Arosio,
    • Daniela Mari,
    • and Jerry W. Shay
    Genome Res. November 2019 29: 1878-1888; Published in Advance September 23, 2019, doi:10.1101/gr.250480.119
    ...and therefore might be considered as a biomarker that mirrors alterations within the human body. Here, we present a new quantitative, highly sensitive droplet digital PCR (ddPCR) method, droplet digital mitochondrial DNA measurement (ddMDM), to measure mtDNA copy number not only from cell populations but also...
    OPEN ACCESS ARTICLE
  2. Method: Application of microdroplet PCR for large-scale targeted bisulfite sequencing
    • H. Kiyomi Komori,
    • Sarah A. LaMere,
    • Ali Torkamani,
    • G. Traver Hart,
    • Steve Kotsopoulos,
    • Jason Warner,
    • Michael L. Samuels,
    • Jeff Olson,
    • Steven R. Head,
    • Phillip Ordoukhanian,
    • Pauline L. Lee,
    • Darren R. Link,
    • and Daniel R. Salomon
    Genome Res. October 2011 21: 1738-1745; Published in Advance July 14, 2011, doi:10.1101/gr.116863.110
    ...-complementarity was restricted by thermodynamic parameters in Primer3 software (Rozen and Skaletsky 2000), and off-target amplification was restricted by electronic PCR (Fig. 1B).Maximumcoveragewas attained at the highest allowable amplicon size with 53°C and 58°C primers affording similar coverage (Supplemental Fig. S2...
  3. Method: Chromosome engineering to correct a complex rearrangement on Chromosome 8 reveals the effects of 8p syndrome on gene expression and neural differentiation
    • Sophia N. Lee,
    • Erin C. Banda,
    • Lu Qiao,
    • Sarah L. Thompson,
    • Karan Singh,
    • Ryan A. Hagenson,
    • Teresa Davoli,
    • Stefan F. Pinter,
    • and Jason M. Sheltzer
    Genome Res. March 2026 36: 547-560; Published in Advance January 12, 2026, doi:10.1101/gr.280425.125
    ...et al. 2010). qPCR confirmed the presence of two copies of the proband-deleted region and two copies of the proband-duplicated region in iPS cells derived from the proband's parents (Fig. 1F). We performed qPCR on the proband cell line at five time points spanning from passage 12 to passage 50...
  4. REVIEW: Advances in PCR-based detection of mycoplasmas contaminating cell cultures.
    • G Rawadi and
    • O Dussurget
    Genome Res. February 1995 4: 199-208
    ..., electron micros- copy, autoradiography, and biochemical assays. ~2'6'1~ Although efforts have fo- cused on the improvement of these tech- niques, detection of mollicutes in cell cultures remains a serious problem. Re- cently, the application of PCR-based methods of detection has attracted much attention...
  5. Research: Xist upstream deletion leads to dysregulation of Xist and autosomal gene expression
    • Sudeshna Majumdar,
    • Lakshmi Sowjanya Bammidi,
    • Hemant C. Naik,
    • Avinchal Manhas,
    • Runumi Baro,
    • Akash Kalita,
    • Amlan Jyoti Naskar,
    • Sundarraj Nidharshan,
    • Girija S. Bariha,
    • Dimple Notani,
    • and Srimonta Gayen
    Genome Res. September 2025 35: 1992-2010; Published in Advance August 6, 2025, doi:10.1101/gr.279822.124
    ...in paternally deleted clonal lines (D1 ΔXp) compared to both WT clones and WT EV clones (log2 FC > 2.4; FDR ≤0.01) (Fig. 2C). Reverse transcription quantitative-PCR (RT-qPCR) analysis, also showed significant upregulation of Xist in paternally deleted clonal lines (D1 ΔXp) compared to the WT EV clones (Fig. 2D...
  6. ARTICLES: Construction of phagemid display libraries with PCR-amplified immunoglobulin sequences.
    • H H Hogrefe and
    • B Shopes
    Genome Res. October 1994 4: S109-S122
    ...Construction of phagemid display libraries with PCR-amplified immunoglobulin sequences. H H Hogrefe and B Shopes Stratagene Cloning Systems, La Jolla, California 92037, USA. Abstract Footnotes Copyright © Cold Spring Harbor Laboratory...
  7. ARTICLE: PCR protocol for DNA recovery from Spurr's-embedded muscle biopsies.
    • F Capon,
    • S L Cicero,
    • G Novelli,
    • and B Dallapiccola
    Genome Res. December 1993 3: 211-212
    ...: Slide PCR in spinal muscular atrophy patients. Mol. Cell. Probes 7 : 221 – 226 . 12.. Spurr, A.R. Spurr, A.R. 1969 . A low-vicosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res. 26 : 31 – 43 . Genome Res Genome Research Genome Res. 1088-9051 Cold Spring Harbor Laboratory...
  8. ARTICLES: In situ localization of PCR-amplified human and viral cDNAs.
    • G J Nuovo,
    • G A Gorgone,
    • P MacConnell,
    • M Margiotta,
    • and P D Gorevic
    Genome Res. November 1992 2: 117-123; doi:10.1101/gr.2.2.117
    ...DNA with incorporation of unlabeled nucleotides (e). The counterstain b--e is nuclear fast red and the blue precipitate signal is the result of the action of alkaline phosphatase on NBT/BCIP. PCR Methods and Applications 119 different; only 1% of the cells had a de- tectable nuclear signal (data not shown). Electron...
  9. METHODS: Loss of Heterozygosity Analysis Using Whole Genome Amplification, Cell Sorting, and Fluorescence-Based PCR
    • Thomas G. Paulson,
    • Patricia C. Galipeau,
    • and Brian J. Reid
    Genome Res. May 1, 1999 9: 482-491; doi:10.1101/gr.9.5.482
    ...electronically, substantially increasing throughput over conventional autoradiographic techniques. Our results validate LOH analysis of flow-purified samples using PEP and subsequent locus-specific PCR with fluorescent-labeled primers and ABI fluorescence detection. Although this approach reduces many...
  10. ARTICLE: Preferential PCR amplification of alleles: mechanisms and solutions.
    • P S Walsh,
    • H A Erlich,
    • and R Higuchi
    Genome Res. May 1992 1: 241-250; doi:10.1101/gr.1.4.241
    ...of the thermal cycler heat block or the actual liquid temperature in a PCR tube can be measured using an electronic ther- mometer and thermocouple. (7) Such measurement provides a check that the thermal cycler is reaching a tempera- ture capable of denaturing all DQa al- leles. The DQc~I.I,4 control DNA pro...
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