Searching journal content for articles similar to Schalkwyk et al. 11 (12): 2142.

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  1. ...Integrated and Sequence-Ordered BAC- and YAC-Based Physical Maps for the Rat Genome Martin Krzywinski 1 , John Wallis 2 , Claudia Gösele 3 , 4 , Ian Bosdet 1 , Readman Chiu 1 , Tina Graves 2 , Oliver Hummel 3 , Dan...
  2. ...(G.G. Bouffard and E.D. Green, unpubl.). Furthermore, STSs developed from the insert ends of several of these BACs have been found to map to orphan YAC contigs, further suggesting that some of the gaps and orphan contigs correspond to regions difficult to clone in YACs. Apparent False...
  3. ...Construction and Validation Overgo-content BAC contigs were constructed according to the hybridization results, using the LANL STS–YAC-derived overgos as the framework for ordering. The overgos from other groups were integrated into the map according to the common BACs hybridized. Finally, 53 contigs were...
  4. ...) and bacterial artificial chromosomes (BACs), that can be used for physical mapping, positional cloning, and sequencing. The publicly available tomato YAC libraries have served as valuable research tools for the isolation of several agriculturally important genes by positional cloning (e.g., Martin et al. 1993...
  5. ...for these experiments was a 0.9-Mb yeast artificial chromosome (YAC) y899G1 and a 1.1-Mb large-insert bacterial clone contig ( Lauer et al., this issue ). View larger version: In this window In a new window Figure 1. Combination genetic, physical, and transcription map of the HH candidate gene region. The first line...
  6. ...libraries [(BAC) Shizuya et al. 1992 ; (PAC) Ioannou et al. 1994 ]. There is a need, therefore, to produce higher density framework maps (15–20 markers per Mb) using RH mapping or YACs. The need is particularly acute for the X chromosome, because neither the whole RH maps nor the genetic maps provide...
  7. .... They may be utilized as an ordered list with gaps defined by the STSs mapped on chromo- some 21 by single-chromosome hybrids, or PFGE. Alternatively, the data may be used to formulate a YAC array and generate a framework graphic map. The accuracy of the latter depends on the use of nonchimeric, stable YAC...
  8. .... These polymorphisms included 105 SSCPs from fully sequenced cDNAs and ESTs (of which 104 were mapped, see below) that defined previously unmapped genes (Fig. 1 ; Table 1 ). We also developed SSCPs to map 10 sequence-tagged sites (STSs) derived from genomic clones in BAC, PAC, and YAC vectors and other sequences...
  9. ...models. High-resolution mapping tools are essential to facilitate high-density mapping. Genetic crosses and clone libraries of yeast, bacterial, and P1-derived artificial chromosomes, respectively (YACs, BACs, PACs), and cosmids all offer routes to high-resolution maps but require screening thousands...
  10. ...-resolution bacterial contig map. The contigs will be sequenced by the chromosome 21 sequencing project within the framework of the German Human Genome Programme. RESULTS Construction of a PAC-Pocket Sublibrary Enriched for the Region A YAC contig from the integrated cosmid-pocket map published previously ( Nizetic et...
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