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  1. ...Dynamic A-to-I RNA editing in response to gut microbiome 1 in honey bees 2 3 Yuange Duan1 #, Min Huang1 #, Lin Ye1 #, Ling Ma1, Yashuai Wu1, Yating Du1, Jiyao Liu1, 4 Fan Song1, Li Tian1, Wanzhi Cai1, Hu Li1, Xin Zhou1, Shiqi Luo1 * 5 6 1. State Key Laboratory of Agricultural and Forestry...
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  2. ...al. 2018).The bulk of exonic A-to-I editing occurs cotranscriptionally before pre-mRNA splicing, as recoding-editing frequently depends on an intronic editing-complementary site (Rodriguez et al. 2012; Hsiao et al. 2018). Here, we systematically study regulation and fine-tuning of editing levels...
  3. ...) from RNA-seq data, which can accurately identify PAS and quantify PAU in a transcriptome-wide manner. Using 3′ end-seq data as the benchmark, we showed that APAIQ outperforms current methods on PAS identification and PAU quantification, including DaPars2, Aptardi, mountainClimber, SANPolyA, and QAPA...
  4. ...authors: Michael.Jantsch@meduniwien.ac.at, Konstantin.Licht@meduniwien.ac.atAbstractPre-mRNA-splicing and adenosine to inosine (A-to-I) RNA-editing occur mostly cotranscriptionally. During A-to-I editing, a genomically encoded adenosine is deaminated to inosine by adenosine deaminases acting on RNA (ADARs...
  5. ...at equal molarity (Supplemental Fig. S1B), minimizing experimental complexity and ligation bias. In contrast, fully modeling GC-content for 50-mers would require 450 permutations, which is impractical. By systematically analyzing GC-content, MFE, and 3D features, we reveal how specific RNA structural...
  6. ...of individual missense A-to-I RNA editing events in cancer development (Chen et al. 2013; Galeano et al. 2013; Han et al. 2014). More recently, we and other groups have systematically characterized the RNA-editing genomic landscape in various cancer types using mRNA-seq data from The Cancer Genome Atlas (TCGA...
  7. ...).To conclude, we found that A-to-I RNA editing promotes the expression of edited genes and pseudogenes in embryos, which is dependent on RNAi.3′ UTR RNA editing is developmentally regulatedThe screen to find editing sites in nonrepetitive regions was performed using transcriptome-wide sequencing of RNA...
  8. ...Te-Lun Mai and Trees-Juen Chuang Genomics Research Center, Academia Sinica, Taipei 11529, Taiwan Corresponding author: trees@gate.sinica.edu.twAbstractAdenosine-to-inosine (A-to-I) RNA editing is a very common co-/posttranscriptional modification that can lead to A-to-G changes at the RNA level...
  9. ...in detecting smaller, specific sets of trans contacts (cliques) that could underlie important local regulations of DNA and RNA biochemistry.In this paper, we overcome this limitation by providing a computational framework that systematically finds sets of jointly interacting loci from Hi-C data. The method...
  10. ...introduce new elements, such as exogenous proteins, activating mutations, plasmids, artificial chromosomes, or knock-in modifications. Conversely, loss-of-function approaches remove specific factors using mutations, siRNA silencing, or gene-editing techniques. These modifications can be applied to either...
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