Searching journal content for articles similar to Rohland et al. 32 (11-12): 2068.

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  1. Research: Ancient dog introgression into the Iberian wolf genome may have facilitated adaptation to human-dominated landscapes
    • Diana Lobo,
    • Hernán E. Morales,
    • Cock Van Oosterhout,
    • José Vicente López-Bao,
    • Pedro Silva,
    • Luis Llaneza,
    • Carolina Pacheco,
    • Diana Castro,
    • Germán Hernández-Alonso,
    • George Pacheco,
    • John Archer,
    • M. Thomas P. Gilbert,
    • Nuno Ferrand,
    • and Raquel Godinho
    Genome Res. March 2025 35: 432-445; Published in Advance February 14, 2025, doi:10.1101/gr.279093.124
    ...sacrificed for this study. Total genomic DNA was extracted using the Qiagen DNeasy blood and tissue kit and quantified on the Qubit DNA quantification system (Thermo Fisher Scientific) using Qubit broad range assay reagents, following the manufacturer's protocol. DNA concentration across all samples...
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  2. Method: HD-Marker: a highly multiplexed and flexible approach for targeted genotyping of more than 10,000 genes in a single-tube assay
    • Jia Lv,
    • Wenqian Jiao,
    • Haobing Guo,
    • Pingping Liu,
    • Ruijia Wang,
    • Lingling Zhang,
    • Qifan Zeng,
    • Xiaoli Hu,
    • Zhenmin Bao,
    • and Shi Wang
    Genome Res. December 2018 28: 1919-1930; Published in Advance November 8, 2018, doi:10.1101/gr.235820.118
    ...DNAHD-Marker assay began with the preparation of biotinylated genomic DNA for in-solution hybridization. For genotyping of SNPs, 1 µg genomic DNA was labeled using a PHOTOPROBE biotin labeling kit (Vector Laboratories) by following the manufacturer's instructions for thermal coupling. For genotyping...
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  3. Method: Copy number variation detection and genotyping from exome sequence data
    • Niklas Krumm,
    • Peter H. Sudmant,
    • Arthur Ko,
    • Brian J. O'Roak,
    • Maika Malig,
    • Bradley P. Coe,
    • NHLBI Exome Sequencing Project,
    • Aaron R. Quinlan,
    • Deborah A. Nickerson,
    • and Evan E. Eichler
    Genome Res. August 2012 22: 1525-1532; Published in Advance May 14, 2012, doi:10.1101/gr.138115.112
    ...of exomes using the CoNIFER software program. First, all of our exomes were captured from genomic DNA that had not undergone whole- amplification (WGA), and we have found that WGA exomes perform very poorly in our analysis. Second, we recommend sequencing exomes to a minimum coverage of 50 million on...
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