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  1. ...in yeast can facilitate the rapid collection of additional clones for gap closure. The same strategy can be also applied for closing the gaps in other complex s. METHODS Construction of the TAR Vectors and Cloning by In Vivo Recombination in Yeast The TAR circularizing vectors, pVC-GAP1, pVC-GAP2, pVC-GAP3...
  2. ...to as “MIC-limited” DNA, are removed during PDE in Paramecium (Bétermier and Duharcourt 2014). At least 25% of the MIC , including DNA repeats (TEs, minisatellites), is eliminated imprecisely, alternatively leading to chromosome fragmentation (with de novo telomere addition to heterogeneous new MAC...
  3. .... Although four chromosomes of the ToxoDB-48_TgME49 contained no telomeric repeat and seven were missing one of the telomeric repeats, all of the chromosome contigs in the TgME49 long-read assembly were assembled up until both telomeric caps (Supplemental Table S5). Both telomeres were found in 12 out...
  4. ...distinct nuclei of T. thermophila using the Hi-C and HiChIP methods. We found that the meiotic crescent MIC has a specific chromosome interaction pattern, with all the telomeres or centromeres on the five MIC chromosomes clustering together, respectively, which is also helpful to identify the midpoints...
  5. ...with short reads. The resultant sequences were further scaffolded using Hi-C data obtained from blood cell nuclei of the same individual (see Methods). The number of output scaffold sequences longer than 1 Mbp (thereafter tentatively designated “chromosomes”) was 50, which closely approximates its chromosome...
  6. ...reassembly.Closing gaps with multiple assembliesEach VC2010 assembly had 76–202 gaps (median, 111 gaps) (Supplemental Table S2). To fill them, we ordered all contigs of the seven assemblies along the six C. elegans nuclear chromosomes and mitochondrial chromosome by aligning them to the N2 reference assembly...
  7. ...chromosome 1q. Genomics 22 : 569 – 578 . ↵ Riethman H. ( 1997 ) Closing in on telomeric closure. Genome Res. 7 : 853 – 855 . ↵ Riethman H.C. , Moyzis R.K. , Meyne J. , Burke D.T. , Olson M.V. ( 1989 ) Cloning human telomeric DNA fragments into Saccharomyces cerevesiae using a yeast artificial chromosome...
  8. ...mechano-electric stimulation of a trigger hair sets the trap to an “attention mode.” In other words, a one touch-induced AP is memorized but does not close the trap. With a second AP, the Dionaea trap closes within a fraction of a second (Forterre et al. 2005; Escalante-Perez et al. 2014), locking...
  9. ...-closingmodule of meraculous, which returns a gap-closing sequence when a unique closure can be inferred by local k-mer walking. Data access The HiRise scaffolder source code used here (version 0.75) is available in the Supplemental Material and hosted on GitHub at https://github.com/DovetailGenomics/HiRise_July2015_GR...
  10. ...is the first coordinate-changing assembly update since 2009; it reflects the resolution of roughly 1000 issues and encompasses modifications ranging from thousands of single base changes to megabase-scale path reorganizations, gap closures, and localization of previously orphaned sequences. We developed a new...
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