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  1. ...the initial list of candidate fusion transcripts of ;96% on average, thus enriching for potentially real fusion transcripts (Supplemental Table S2). Also, similar to Table 1. Summary statistics of data generation with next-generation RNA sequencing Sample Tissue type ETS fusion No. of lanes Read length No...
  2. ...Next-generation DNA sequencing of paired-end tags (PET) for transcriptome and genome analyses Melissa J. Fullwood 1 , 2 , Chia-Lin Wei 1 , Edison T. Liu 1 , 3 and Yijun Ruan 1 , 4 , 5 1 Genome Institute of Singapore, Agency...
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  3. ...techniques have been developed that monitor transcription as it occurs. Pioneering work introduced methods such as global run-on sequencing (GRO-seq) (Core and Lis 2008) and precision nuclear run-on sequencing (PRO-seq) (Kwak et al. 2013), which measure the location and activity of engaged RNA polymerase II...
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  4. ...cells in which the tagging element has inserted into an actively transcribed gene. A new and highly sensitive reporter system has recently been described and is well suited for gene tagging. A nontoxic fluorescent substrate of β-lactamase, CCF2-AM, enables real-time and sensitive monitoring...
  5. ...operations. The LI-COR, Inc. (Lincoln, NE) ( http://www.licor.com ) Model 4200 (IR2) automated DNA sequencer, launched in 1997, is a two-dye near-infrared DNA analysis system. It can detect the products of two different sequencing reactions in parallel enabling pooling reactions and simultaneous...
  6. ...and increased upon shRNA-mediated silencing (Supplemental Fig. S2A). TRAP1 silencing with two different siRNA sequences in both HeLa and HT29 colorectal carcinoma cells yielded analogous results in fluorescent noncanonical amino acid tagging (FUNCAT) assays (Supplemental Fig. S2B).View larger version...
  7. ...oligonucleotide (ASO) technology (Roux et al. 2017). We then quantified the effect of each knockdown on cell growth and morphology using real-time imaging, followed by Cap Analysis Gene Expression (CAGE) (Murata et al. 2014) deep sequencing to reveal molecular pathways associated with each lncRNA. In contrast...
  8. ...et al. 1999 ). F-PEC data could augment tissue microarray analysis, in particular when an antibody or in situ hybridization assay is not readily available for a particular gene. Regardless of the follow-up approach, there is a real need to be able to rapidly assess well-documented samples...
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