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LETTER: Isolation of the repertoire of VSG expression site containing telomeres of Trypanosoma brucei 427 using transformation-associated recombination in yeast
- Marion Becker,
- Niall Aitcheson,
- Elaine Byles,
- Bill Wickstead,
- Edward Louis,
- and Gloria Rudenko
Genome Res. November 2004 14: 2319-2329; doi:10.1101/gr.2955304
...) as recombinational target ( Fig. 1A ). Yeast cotransformation of vector pEB4 containing the target and a single yeast telomere located at one end of the linearized vector, and T. brucei 427 genomic DNA resulted in cloning of trypanosome telomeres as linear half YACs ( Fig. 1B ). In three independent experiments...
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LETTER: A High-Resolution Physical Map of Human Chromosome 21p Using Yeast Artificial Chromosomes
- Sheng-Yue Wang,
- Marc Cruts,
- Jurgen Del-Favero,
- Yi Zhang,
- Fadel Tissir,
- Marie-Claude Potier,
- David Patterson,
- Dean Nizetic,
- Assumpció Bosch,
- Haiming Chen,
- Lynda Bennett,
- Xavier Estivill,
- Anna Kessling,
- Stylianos E. Antonarakis,
- and Christine van Broeckhoven
Genome Res. November 1, 1999 9: 1059-1073; doi:10.1101/gr.9.11.1059
...sequence tags (ESTs). Furthermore, we used these markers to construct a yeast artificial chromosome (YAC) contig map of chromosome 21p ranging from the centromere to the rDNA gene cluster at 21p12. The integrity of the YAC contig map was validated by fluorescence in situ hybridization (FISH) analyses...

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