Searching journal content for articles similar to Rashtchian 4 (2): S83.

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  1. ...change and dispersion for RNA-seq data with DESeq2. Genome Biol 15: 550. doi:10.1186/s13059-014-0550-8 ↵Maniatis T, Kee SG, Efstratiadis A, Kafatos FC. 1976. Amplification and characterization of a beta-globin gene synthesized in vitro. Cell 8: 163–182. doi:10.1016/0092-8674(76)90001-5 ↵Marco A. 2018...
  2. ...Dynamics and consequences of differential RNA isoform production during cardiomyocyte 1 fate determination and early-stage maturation 2 3 Joanna Delimata-Raczek1, Natalia Koralewska1, Bart Krist1, Magdalena Rakoczy1, Marek 4 Figlerowicz1, Ireneusz Stolarek1* 5 6 Institute of Bioorganic Chemistry...
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  3. ...sequencing by replacing the standard DNA polymerase with a reverse transcriptase (Vilfan et al. 2013), although this approach has not been widely adopted. Nanopore direct RNA sequencing has made it possible to directly sequence full-length native RNA molecules without reverse transcription and amplification...
  4. ...sites by Sanger sequencing For the p.482 Ile >Met editing site in Adar, we conducted amplification ofAdar from both gDNA and cDNA extracted from the same sample (the first batch data of three CV and three GF). For cDNA synthesis, 1 µg of total RNA was incubated with gDNA wiper (Vazyme Biotech) for 2 min...
  5. ...of Pharmaceutical Biotechnology and Department of Medicine, the University of Hong Kong, Hong Kong SAR, China ↵9 These authors contributed equally to this work. Corresponding authors: su@chemie.uni-siegen.de, zhouxm@scnu.edu.cn, qz.lian@siat.ac.cnAbstractRNA sequencing (RNA-seq) is a pivotal tool for transcriptomic...
  6. ...for polymerase chain reaction (PCR) amplification and cDNA synthesis steps, thus preserving the native state of RNA molecules and providing a less biased and comprehensive view of the transcriptome (Workman et al. 2020).However, key challenges persist, such as the lack of commercial barcoding kits...
  7. ....beerenwinkel@bsse.ethz.chAbstractIn cancer, genetic and transcriptomic variations generate clonal heterogeneity, leading to treatment resistance. Long-read single-cell RNA sequencing (LR scRNA-seq) has the potential to detect genetic and transcriptomic variations simultaneously. Here, we present LongSom, a computational workflow leveraging...
  8. ...rearrangements offers several advantages to DNA-based methods. The use of RNA transcripts to uncover mutation events leverages the transcriptional amplification of these genomic events that may improve detection of rare events. Finally, it would seem reasonable to exploit all of the biological inferences...
  9. ...with hallmarks of both, a 5′ CAP and a poly(A) tail. It has been reported that the TeloPrime Full-Length cDNA Amplification Kit could selectively synthesize cDNA molecules from mRNAs carrying a 5′ CAP, in complement to the standard PacBio cDNA library preparation protocol that could only selectively synthesize c...
  10. ...: arturo.mari-ordonez@gmi.oeaw.ac.atAbstractA handful of model plants have provided insight into silencing of transposable elements (TEs) through RNA-directed DNA methylation (RdDM). Guided by 24 nt long small-interfering RNAs (siRNAs), this epigenetic regulation installs DNA methylation and histone...
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