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METHODS: Construction of Long-Transcript Enriched cDNA Libraries from Submicrogram Amounts of Total RNAs by a Universal PCR Amplification Method
- Yulan Piao,
- Naomi T. Ko,
- Meng K. Lim,
- and Minoru S.H. Ko
Genome Res. September 1, 2001 11: 1553-1558; doi:10.1101/gr.185501
...L of purified DNAs were amplified by long-range, high-fidelity PCR using 0.25 μL (5 units/μL) of Ex Taq polymerase (Takara) with 20 pmol of PCR primer (Primer L, 5′-GTCATCTATGTCGGGTGGTCGACAAGAGGTAATCC-3′ or Primer S, 5′-GTCATCTATGTCGGGTG-3′) in a total 50 μL reaction. The amplification cycles were 25 cycles...
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Resource: Chimeric 16S rRNA sequence formation and detection in Sanger and 454-pyrosequenced PCR amplicons
- Brian J. Haas,
- Dirk Gevers,
- Ashlee M. Earl,
- Mike Feldgarden,
- Doyle V. Ward,
- Georgia Giannoukos,
- Dawn Ciulla,
- Diana Tabbaa,
- Sarah K. Highlander,
- Erica Sodergren,
- Barbara Methé,
- Todd Z. DeSantis,
- The Human Microbiome Consortium,
- Joseph F. Petrosino,
- Rob Knight,
- and Bruce W. Birren
Genome Res. March 2011 21: 494-504; Published in Advance January 6, 2011, doi:10.1101/gr.112730.110
...diversity among environmental samples is commonly assessed with PCR-amplified 16S rRNA gene (16S) sequences. Perceived diversity, however, can be influenced by sample preparation, primer selection, and formation of chimeric 16S amplification products. Chimeras are hybrid products between multiple parent...
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ARTICLES: Interference of PCR amplification by the polyamines, spermine and spermidine.
- H Ahokas and
- M J Erkkilä
Genome Res. August 1993 3: 65-68
...the specificity of PCR. Nucleic Acids Res. 18 : 7465 . 16.. Henry, R.J. , Oono. K. Henry, R.J. and Oono. K. 1991 . Amplification of a GC-rich sequence from barley by a two-step polymerase chain reaction in glycerol. Plant Mol. Biol. Rep. 9 : 139 – 144 . 17.. Chou, Q., , Russell, M. , Birch, D.E. , Raymond, J...
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ARTICLES: Amplimers with 3'-terminal phosphorothioate linkages resist degradation by vent polymerase and reduce Taq polymerase mispriming.
- C M de Noronha and
- J I Mullins
Genome Res. November 1992 2: 131-136; doi:10.1101/gr.2.2.131
.... Abstract The 3'--&gt;5' exonuclease activity of Vent, a thermostable polymerase from Thermococcus litoralis, enhances DNA replication fidelity but also diverts PCR primers (amplimers) from targeted amplification by degrading their 3' termini. We demonstrate that amplimers with a 3-base 3'-terminal...
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METHODS: SNP Genotyping by Multiplexed Solid-Phase Amplification and Fluorescent Minisequencing
- Michael H. Shapero,
- Kerstin K. Leuther,
- Anhthu Nguyen,
- Melissa Scott,
- and Keith W. Jones
Genome Res. November 1, 2001 11: 1926-1934; Published in Advance October 15, 2001, doi:10.1101/gr.205001
...the same instrument setup to detect total released PCR product in all lanes. Four-Color SNP Sequencing Following solid-phase amplification reactions, beads were equilibrated into 1× NEBuffer 2 and digested overnight at 37°C with 40 U Stu I restriction enzyme. Beads were washed once with fresh 1× NEBuffer 2...
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ARTICLES: Generation of entire human papillomavirus genomes by long PCR: frequency of errors produced during amplification.
- A C Stewart,
- P E Gravitt,
- S Cheng,
- and C M Wheeler
Genome Res. August 1995 5: 79-88; doi:10.1101/gr.5.1.79
...amplification, restriction fragment length polymorphisms, nucleotide sequence and, phylogenetic algorithms. J. Infect. Dis. 170 : 1077 – 1085 . Chou, Q., , Russell, M. , Birch, D.E. , Raymond, J. , Bloch. W. Chou, Q., Russell, M. Birch, D.E. Raymond, J. and Bloch. W. 1992 . Prevention of pre-PCR mis-priming...
ARTICLE: Wax-embedded PCR reagents.
- P Blair,
- R Ramanujam,
- and B A Burdick
Genome Res. December 1994 4: 191-194
..., J. and Bloch. W. 1992 . Prevention of pre-PCR mis-priming and primer dimerization improves low-copy-number amplifications. Nucleic Acids Res. 20 : 1717 – 1723 . 7.. Bassam, B.J. , Caetano-Anolles. G. Bassam, B.J. and Caetano-Anolles. G. 1993 . Automated “Hot Start” PCR using mineral oil and paraffin...
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ARTICLE: Optimization and troubleshooting in PCR.
- K H Roux
Genome Res. April 1995 4: S185-S194
...amplification. Nucleic Acids Res. 19 : 4008 . 13. Erlich, H.A., Gelfand, D. and Sninsky. J.J. 1991 . Recent advances in the polymerase chain reaction. Science 252 : 1643 – 1651 . 14. Mullis, K.B. 1991 . The polymerase chain reaction in an anemic mode: How to avoid cold oligodeoxyribonuclear fusion. PCR Methods...
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REVIEW: Empirical aspects of strand displacement amplification.
- G T Walker
Genome Res. August 1993 3: 1-6
...the need for instrumented temperature cycling as with PCR and the ligase chain reaction. Highly reproducible temperature profiles, over a large array of samples, can burden the accuracy and expense of an amplification technique. However, the expense of a temperature cycler is offset somewhat by the cost...
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METHODS: High-Throughput SNP Allele-Frequency Determination in Pooled DNA Samples by Kinetic PCR
- Søren Germer,
- Michael J. Holland,
- and Russell Higuchi
Genome Res. February 1, 2000 10: 258-266; doi:10.1101/gr.10.2.258
...-time quantitative) PCR with allele-specific amplification and requires no post-PCR processing. The relative amounts of each allele in a sample are quantified. This is performed by dividing equal aliquots of the pooled DNA between two separate PCR reactions, each of which contains a primer pair specific to one...

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