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  1. ...nucleosomes was collected (S1 fraction). Nucleosomes in the remaining insoluble fraction were extracted by rotating in 300 µL Nucleosome Release Buffer (10 mM Tris [pH 7.5], 10 mM NaCl, 0.2 mM EDTA, with 1× cOmplete EDTA-free Protease Inhibitor Cocktail [Roche] and 10 mM sodium butyrate) for 1 h at 4°C...
  2. ...that a single catalytic subunit in rat and chicken calpains possesses a full bona fide proteolytic activity ( Yoshizawa et al. 1995 ) reinforces the potential processing capacity of the putative plasmodial calpain. View larger version: In this window In a new window Figure 1. Multiple alignment of the catalytic...
  3. ..., dissected, and then frozen with liquid nitrogen. The cerebella from multiple rats from the same age group were homogenized together in 0.32 M sucrose, 4 mM HEPES, and protease inhibitors. After a BCA protein assay (Pierce), each developmental cerebellar homogenate was mixed in a 1:1 (w/w) ratio with 15N...
  4. ...., Szpirer, C., Levan, G., and Le Cam, A. 1990 . Primary structure and assignment to chromosome 6 of three related rat genes encoding liver serine protease inhibitors. Gene 94 : 273 -282. ↵ Pickeral, O.K., Makalowski, W., Boguski, M.S., and Boeke, J.D. 2000 . Frequent human genomic DNA transduction driven...
  5. ...Andrew S Greene Jennifer Mingyu, Liang Isaac R, Matus I Matus Greene genome;gr.6587008 10.1101/gr.6587008 1088-9051 MicroRNA–target pairs in the rat kidney identified by microRNA microarray, proteomic, and bioinformatic analysis MicroRNA–target pairs in the rat kidney identified by microRNA microarray...
  6. .... ( 1984 ) Polyclonal IgE increase after HgCl2 injections in BN and LEW rats: A genetic analysis. Immunogenetics 20 : 227 – 236 . ↵ Sarafino E.P. , Goldfedder J. ( 1995 ) Genetic factors in the presence, severity, and triggers of asthma. Arch. Dis. Child. 73 : 112 – 116 . ↵ Schena M. , Shalon D. , Davis R...
  7. ...summary of the regulatory elements identified to date across the mouse Tal1 locus Mouse Tal1 array Figure 2A shows the annotation of the Stil (formerly known as Sil ), Tal1 , and Pdzk1ip1 (formerly known as Map17 ) genes above the UCSC mouse/rat/human/dog/chicken conservation score derived using the Multi...
  8. ...sites that are consistent with known functions. A yeast spliceosome component, the U1 snRNP, localized preferentially to intron-containing genes, while the RNA exonuclease Rat1p localized to the 3′ ends of genes where it is involved in transcription termination as well as mRNA degradation ( Kotovic et...
  9. ..., M B Zarbl H Zarbl Zarbl, H Bahramian, MB H, Zarbl Bahramian, M.B genome;4/3/145 1088-9051 Direct gene quantitation by PCR reveals differential accumulation of ectopic enzyme in rat-1 cells, v-fos transformants, and revertants. Direct gene quantitation by PCR reveals differential accumulation...
  10. ..., contains a number of stop codons and is presumably the 5′ untranslated region (UTR). Additional evidence for this was revealed by database searches that identified a highly significant match to an expressed sequence tag (EST) containing the 5′ end of a rat incisor cDNA clone (GenBank accession no. R47026...
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