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Method: Sequence-targeted nucleosome sliding in vivo by a hybrid Chd1 chromatin remodeler
- Jeffrey N. McKnight,
- Toshio Tsukiyama,
- and Gregory D. Bowman
Genome Res. May 2016 26: 693-704; Published in Advance March 18, 2016, doi:10.1101/gr.199919.115
...for endogenous factors should be reduced -wide. In addition to targeting through DNA binding preferences of endogenous factors, combining Chd1 with designed targeting systems used previously with chromatin modifiers—such as TALE, engineered zinc fingers, and CRISPR/Cas9 (Mendenhall et al. 2013; Keung et al. 2014...
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Perspective: Enabling functional genomics with genome engineering
- Isaac B. Hilton and
- Charles A. Gersbach
Genome Res. October 2015 25: 1442-1455; doi:10.1101/gr.190124.115
...and TALEs, in which protein moieties dictate DNA recognition, CRISPR/Cas systems utilize RNA-mediated Watson-Crick bonding for recognition of nucleic acids. Prokaryotes harboring type II CRISPR/Cas systems transcribe CRISPR-RNAs (crRNAs) that hybridize with trans-activating crRNAs (tracrRNAs) that complex...
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