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  1. ...Indels, structural variation, and recombination drive genomic diversity in Plasmodium falciparum Alistair Miles 1 , 2 , Zamin Iqbal 3 , Paul Vauterin 1 , Richard Pearson 1 , 2 , Susana Campino 2 , Michel Theron 2 , Kelda...
  2. ...In a new window Figure 1. Partial alignment of the ookinete adhesive protein between the Plasmodium falciparum (PLAFA) and the rodent malaria agent Plasmodium berghei (PLABE) sequences, showing two low-complexity elements (lower case, unaligned) between regions of alignment (upper case). The first region...
  3. ...to the equivalent 18S/28S rRNA Chr 1 pileups. Regions with pileups on inner portions of Chr 5, 7, and 8 are low complexity regions composed by tandem repeats (Supplemental Table S8). In Chr 5, we have one uncharacterized protein (CPATCC_0023030), full of tandem repeats and good RNA-seq support for its expression...
  4. ...% of the . These sequences were searched for similarity against the other Plasmodium s using WU-BLASTN ( http://blast.wustl.edu/ ) with dust and seg low-complexity filters. Only hits with a length ≥50 bp and with at least 70% identity were further processed. BLAST-matches were mapped back at the P. falciparum and merged...
  5. ...(Mmus), chicken (Ggal), Fugu (Frub), Ciona (Cint), fruit fly (Dmel), C. elegans (Cele), Arabidopsis (Atha), and Plasmodium (Pfal) (for simplicity only one rodent, one teleost, and one insect are shown). Amino-acid positions of each domain within the protein are also indicated in the domain...
  6. ...highlight several interesting anomalies. Drosophila melanogaster possesses an overabundance of poly-Q RCPs, >3.5-fold more than that of Homo sapiens and sixfold more than another insect, the mosquito Anopheles gambiae ( Fig. 3B ). In contrast, poly-Q repeats are extremely rare in Plasmodium falciparum...
  7. ...and evaluate the approach using extensive simulations and use it to characterize the rate and spectrum of de novo mutation events in 119 progeny from four Plasmodium falciparum experimental crosses, using long-read data on the parents to inform reconstructions of the progeny and to detect several known...
  8. ...1 and SURFIN families, respectively. Next, we searched for those predicted motifs in all predicted proteins (excluding low-complexity regions) of the 11 sequenced Plasmodium species and visualized the results as a binary occurrence matrix (Supplemental Fig. S14B). Although some proteins share...
  9. ...Bac mutants in the human malaria parasite Plasmodium falciparum but should be applicable to many other eukaryotic genomes. QIseq proved accurate, confirming known sites in >100 mutants, and sensitive, identifying and monitoring sites over a >10,000-fold dynamic range of sequence counts. Applying QIseq...
  10. .....org Nucleosome positioning in Plasmodium protein-free DNA than histone-bound regions and can be accessed by any type of DNA-binding proteins during most of the erythrocytic cycle, especially at the trophozoite and early schizont stages (18–30 hpi). We verified that this abundance of protein-free regions...
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