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  1. ..., and TMPRSS2 ) and 14 new additional gene signatures consisting of cDNA selection products and ESTs. Forthcoming genomic sequence information will unravel the structural organization of potential candidate genes involved in specific features of Down syndrome pathogenesis. Footnotes ↵ 1...
  2. ...Abstract Cot-based cloning and sequencing (CBCS) is a powerful tool for isolating and characterizing the various repetitive components of any genome, combining the established principles of DNA reassociation kinetics with high-throughput sequencing. CBCS was used to generate sequence libraries...
  3. ...element-related genes in the rice genome in the form of a rice expressed sequence tag, full-length cDNA, or plant homolog from our comparative analyses could be found. Although the majority of the putative homologs were obtained from Poaceae species, putative homologs were identified in dicotyledonous...
  4. ...of Eschericia coli is essential: Application to the polA gene. J. Bacteriol. 158 : 636 – 643 . ↵ Kimmel B. , Palazzola M.J. , Martin C. , Boeke J.D. , Devine S.E. Green E. , Birren B. , Myers R. , Hieter P. ( 1997 ) Transposon-mediated DNA sequencing. in Genome analysis: A laboratory manual , eds Green E...
  5. ...a different problem than selecting oligonucleotides for gene-based arrays, primarily because end-to-end or overlapping tile layouts present fewer options with regard to sequence selection. A number of factors should be considered when tiling genomic DNA with oligonucleotides, including tiling resolution...
  6. ...for technology that can map breakpoints of these aberrations to delineate genes involved in a syndrome. The ability of SNP-CGH to simultaneously measure both intensity differences and allelic ratios in a genomic sample allows both DNA copy number and copy-neutral LOH to be assessed. This is particularly...
  7. ...percentage of human genes have been cloned and mapped. To facilitate more rapid gene mapping and disease gene isolation, chromosome 5-specific cDNA libraries have been constructed from five sources. DNA sequencing and regional mapping of 205 unique cDNAs indicates that 25 are from known chromosome 5 genes...
  8. .... 2003 . Array-based comparative genomic hybridization for -wide screening of DNA copy number in bladder tumors. Cancer Res. 63 : 2872 -2880. ↵ Yang, L., Tran, D.K., and Wang, X. 2001 . BADGE, Beads array for the detection of gene expression, a high-throughput diagnostic bioassay. Genome Res. 11 : 1888...
  9. ...(Supplemental Fig. S1). In mice carrying HACs, some progenitor cells lost the ectopic chromosome and only a proportion of the retinal clones still harbored HACs (Fig. 3A). When visualized by fluorescence in situ hybridization (FISH) using whole human genomic DNA or human Cot1 as a probe, both L-HAC and C...
  10. ...the same gene can exhibit different levels of polyadenylation. Canonical transcripts were also analyzed for all genes shown,with the exception of RERE, andwere expressed in all cDNAs (data not shown). Most genes that exhibit PTES produce multiple products The high-throughput sequencing identified 17 genes...
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