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Peterson 12 (2): 217
.
Displaying results 1-10 of
57
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Method
:
Agonist-induced functional analysis and cell sorting associated with single-cell transcriptomics characterizes cell subtypes in normal and pathological brain
Sara Castagnola
,
Julie Cazareth
,
Kevin Lebrigand
,
Marielle Jarjat
,
Virginie Magnone
,
Sébastien Delhaye
,
Frederic Brau
,
Barbara Bardoni
,
and
Thomas Maurin
Genome Res.
November 2020
30
:
1633
-
1642
;
Published in Advance
September 24, 2020
,
doi:
10.1101/gr.262717.120
...
brain
, a highly heterogeneous tissue, enriching it in interneurons by stimulation with KCl or with AMPA, an agonist of the glutamate receptors, followed by sorting based on calcium levels. After AMPA stimulus, single-cell transcriptomics of these aiFACS-selected interneurons resulted in a nine...
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Method
:
Spatial domain detection using contrastive self-supervised learning for spatial multi-omics technologies
Jianing Yao
,
Jinglun Yu
,
Brian Caffo
,
Stephanie C. Page
,
Keri Martinowich
,
and
Stephanie C. Hicks
Genome Res.
July 2025
35
:
1621
-
1632
;
Published in Advance
May 20, 2025
,
doi:
10.1101/gr.279380.124
...and reduce noise, we established a threshold of six standard deviations above the mean
pixel
values and applied a max filter with a 10 × 10 image box to facilitate the detection of protein-rich regions by Proust.DLPFC Visium SPGThe human DLFPC
brain
tissue was measured on the 10x Genomics Visium SPG platform...
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Method
:
Tissular chromatin-state cartography based on double-barcoded DNA arrays that capture unloaded PA-Tn5 transposase
Maria Grazia Mendoza-Ferri
,
Gwendoline Lozachmeur
,
Maximilien Duvina
,
Laetitia Perret
,
Didier Merciris
,
Anne Gigout
,
and
Marco Antonio Mendoza-Parra
Genome Res.
July 2025
35
:
1633
-
1645
;
Published in Advance
May 13, 2025
,
doi:
10.1101/gr.280305.124
...spatial epigenomic element (SpExel), in analogy to picture elements (
pixels
). (G) Digital display corresponding to the number of adjusted read counts associated to the physical positions in the mouse
brain
section (heatmap displayed in logarithmic scale). (H) Venn diagram displaying the number...
Abstract
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Method
:
A novel multislice framework for precision 3D spatial domain reconstruction and disease pathology analysis
Daijun Zhang
,
Ren Qi
,
Xun Lan
,
and
Bin Liu
Genome Res.
August 2025
35
:
1794
-
1808
;
Published in Advance
July 14, 2025
,
doi:
10.1101/gr.280281.124
...but lack SC resolution owing to spot-level sequencing. Advanced technologies like Stereo-seq (Chen et al. 2022), Visium HD (Nagendran et al. 2023),
Pixel
-seq (Fu et al. 2022), and Seq-Scope (Cho et al. 2021) can achieve subcellular resolution and potentially enable SC analysis through cell segmentation...
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Method
:
Chromosome engineering to correct a complex rearrangement on Chromosome 8 reveals the effects of 8p syndrome on gene expression and neural differentiation
Sophia N. Lee
,
Erin C. Banda
,
Lu Qiao
,
Sarah L. Thompson
,
Karan Singh
,
Ryan A. Hagenson
,
Teresa Davoli
,
Stefan F. Pinter
,
and
Jason M. Sheltzer
Genome Res.
March 2026
36
:
547
-
560
;
Published in Advance
January 12, 2026
,
doi:
10.1101/gr.280425.125
...were changed every day until D18. At D18, cultures were enzymatically passaged with Accutase (EMD Millipore SCR005) onto poly-D lysine and laminin-coated substrates at 2.1 × 104 cells/cm2 into ND media: neurobasal media, B27, GlutaMAX, NEAA, penicillin–streptomycin supplemented with
brain
...
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Method
:
Integration of hyperspectral imaging and transcriptomics from individual cells with SpectralSeq
Yike Xie
,
Abbas Habibalahi
,
Ayad G. Anwer
,
Kanu Wahi
,
Jacqueline Bailey
,
Francis Lin
,
Catherine Gatt
,
Emma M.V. Johansson
,
Tatyana Chtanova
,
Jeff Holst
,
Ewa Goldys
,
and
Fabio Zanini
Genome Res.
August 2025
35
:
1809
-
1820
;
Published in Advance
July 8, 2025
,
doi:
10.1101/gr.280014.124
...
pixels
within the segmentation mask were averaged. (D) Total reads per cell versus percentage of mitochondrial over total reads for experimental batch 1, with the same cell as in B highlighted as a red star. (E) As in D but for experimental batch 2. (F) Hyperspectral PCA for both batches combined...
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Method
:
Joint imputation and deconvolution of gene expression across spatial transcriptomics platforms
Hongyu Zheng
,
Hirak Sarkar
,
and
Benjamin J. Raphael
Genome Res.
December 2025
35
:
2734
-
2743
;
Published in Advance
November 17, 2025
,
doi:
10.1101/gr.280555.125
Abstract
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Review
:
A systematic guide for identifying transcription factors that directly regulate the expression of a gene of interest
Andrew D. Bates
,
Dawid Grzela
,
Maciej Studzian
,
Louise Brennan
,
Moli Williams
,
Conor Fawcett
,
Beth Hammond
,
Manreen Grewal
,
Marcin Ratajewski
,
Lukasz Pulaski
,
and
Urszula L. McClurg
Genome Res.
March 2026
36
:
433
-
459
;
Published in Advance
February 17, 2026
,
doi:
10.1101/gr.281154.125
Abstract
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ARTICLE
:
Multiplex Three-Dimensional Brain Gene Expression Mapping in a Mouse Model of Parkinson's Disease
Vanessa M. Brown
,
Alex Ossadtchi
,
Arshad H. Khan
,
Simon Yee
,
Goran Lacan
,
William P. Melega
,
Simon R. Cherry
,
Richard M. Leahy
,
and
Desmond J. Smith
Genome Res.
June 1, 2002
12
:
868
-
884
;
Published in Advance
May 16, 2002
,
doi:
10.1101/gr.229002
...Multiplex Three-Dimensional
Brain
Gene Expression Mapping in a Mouse Model of Parkinson's Disease Vanessa M. Brown 1 , 2 , Alex Ossadtchi 3 , Arshad H. Khan 1 , 2 , Simon Yee 1 , Goran Lacan 1 , William P. Melega 1 , Simon...
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Resource
:
Comparing genomic and epigenomic features across species using the WashU Comparative Epigenome Browser
Xiaoyu Zhuo
,
Silas Hsu
,
Deepak Purushotham
,
Prashant Kumar Kuntala
,
Jessica K. Harrison
,
Alan Y. Du
,
Samuel Chen
,
Daofeng Li
,
and
Ting Wang
Genome Res.
May 2023
33
:
824
-
835
;
Published in Advance
May 8, 2023
,
doi:
10.1101/gr.277550.122
...at the nucleotide level with more than 10
pixels
per nucleotide. The sequence strand in the alignment is illustrated using arrows. Syntenic nucleotides from hg38 and mm10 are vertically aligned with gaps inserted. Matching nucleotides are connected using a short vertical line in the alignment track. (B) Displaying...
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March 2026, 36 (3)
In This Issue
Single-cell gene signature scoring
Chromosome engineering in 8p syndrome
Convergent evolution in arid rodents
Short tandem repeat genotyping using long reads
Spectral component approach for recent population structure
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