Searching journal content for articles similar to Ohler et al. 3 (2): 115.

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  1. Methods: DNA Mapping Using Microfluidic Stretching and Single-Molecule Detection of Fluorescent Site-Specific Tags
    • Eugene Y. Chan,
    • Nuno M. Goncalves,
    • Rebecca A. Haeusler,
    • Amie J. Hatch,
    • Jonathan W. Larson,
    • Anthony M. Maletta,
    • Gregory R. Yantz,
    • Eugene D. Carstea,
    • Martin Fuchs,
    • Gordon G. Wong,
    • Steven R. Gullans,
    • and Rudolf Gilmanshin
    Genome Res. June 2004 14: 1137-1146; doi:10.1101/gr.1635204
    ...spot ExIII was displaced by a distance Z down the channel ( Fig. 1A ). Typical emission signals detected in data channels 1–3 are presented in Figure 1C . The DNA backbone was stained with the intercalating dye, TOTO-3, which was excited by red light (633 nm) and emitted fluorescence when DNA traveled...
  2. ARTICLE: Long-distance PCR.
    • O S Foord and
    • E A Rose
    Genome Res. June 1994 3: S149-S161
    ...Applic. 2 : 51 – 59 . 4.. Ohler, L., , Zollo, M. , Mansfield, E. , Rose. E.A. Ohler, L., Zollo, M. Mansfield, E. and Rose. E.A. 1993 . Use of a sensitive fluorescent intercalating dye to detect PCR products of low copy number and high molecular weight. PCR Methods Applic. 3 : 85 – 140 . 5.. Shizuya, H...
  3. METHODS: Self-Reporting PNA/DNA Primers for PCR Analysis
    • Mark J. Fiandaca,
    • Jens J. Hyldig-Nielsen,
    • Brian D. Gildea,
    • and James M. Coull
    Genome Res. April 1, 2001 11: 609-613; doi:10.1101/gr.170401
    ...method that we have developed for homogeneous detection of PCR products is suitable for both realtime and endpoint analysis. It utilizes a generic Q-PNA reagent to quench fluorescent signal from nonincorporated PCR primers. In cases where endpoint PCR is sufficient to analyze samples of interest...
  4. ARTICLE: Improved quantitative PCR using nested primers.
    • L A Haff
    Genome Res. June 1994 3: 332-337
    ...implementat ion of intercalat- ing dye detection is its lack of specificity. Unless the PCR is very specific, nonspe- cific products are quantitated together with specific products, and a false esti- mation of initial copy number may be obtained. This is particularly a problem for amplifications starting...
  5. ARTICLES: Fluorescence-based RT PCR analysis: determination of the ratio of soluble to membrane-bound forms of Fc gamma RIIA transcripts in hematopoietic cell lines.
    • M A Keller,
    • D L Cassel,
    • E F Rappaport,
    • S E McKenzie,
    • E Schwartz,
    • and S Surrey
    Genome Res. August 1993 3: 32-38
    ...for determination of the ratio of two alternatively spliced transcripts in different cell types. Fluorescence detection, by an automated DNA sequencer, allows enhanced sensitivity and ease of data processing. PCR products are fluorescently tagged using a dye-labeled oligonucleotide primer during the PCR reaction...
  6. Review: Leveraging the power of long reads for targeted sequencing
    • Shruti V. Iyer,
    • Sara Goodwin,
    • and William Richard McCombie
    Genome Res. November 2024 34: 1701-1718; doi:10.1101/gr.279168.124
    ...the overall efficiency of this tool.Summary of CRISPR-Cas9-based enzymatic targeting approachesCas9-based approaches are more sensitive than PCR and hybridization approaches as they rely on short (20–30 nt) sequences of complementarity and a PAM sequence for cut site detection; therefore, mismatches...
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  7. ARTICLE: Metastable single-strand DNA conformational polymorphism analysis results in enhanced polymorphism detection.
    • T Kasuga,
    • J Cheng,
    • and K R Mitchelson
    Genome Res. February 1995 4: 227-233
    ...SSCP) represents a novel and sensitive system for detection of sequence variation between closely related DNAs. The technique used here for the preparation of the purified ssDNAs is potentially useful for automated PCR-SSCP analysis using capillary electrophoresis or other methods. Footnotes Copyright © Cold...
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