Searching journal content for articles similar to O'Hara et al. 29 (11): 1878.

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  1. Method: High-throughput single-cell functional elucidation of neurodevelopmental disease–associated genes reveals convergent mechanisms altering neuronal differentiation
    • Matthew A. Lalli,
    • Denis Avey,
    • Joseph D. Dougherty,
    • Jeffrey Milbrandt,
    • and Robi D. Mitra
    Genome Res. September 2020 30: 1317-1331; Published in Advance September 4, 2020, doi:10.1101/gr.262295.120
    .... 2018; Sanson et al. 2018; Xie et al. 2018). We validated the efficacy of repression for two gRNAs targeting each of six candidate genes using quantitative real-time PCR (qRT-PCR) in LUHMES neural progenitor cells constitutively expressing dCas9-KRAB. All tested gRNAs induced significant down...
  2. Review: A systematic guide for identifying transcription factors that directly regulate the expression of a gene of interest
    • Andrew D. Bates,
    • Dawid Grzela,
    • Maciej Studzian,
    • Louise Brennan,
    • Moli Williams,
    • Conor Fawcett,
    • Beth Hammond,
    • Manreen Grewal,
    • Marcin Ratajewski,
    • Lukasz Pulaski,
    • and Urszula L. McClurg
    Genome Res. March 2026 36: 433-459; Published in Advance February 17, 2026, doi:10.1101/gr.281154.125
    ...); this cDNA template is used for the quantitative PCR or real-time PCR reaction (qRT-PCR). Specific target fluorescent DNA-binding probes or fluorescent dsDNA-binding dyes (nonspecific used with target-specific primers) are incorporated during the PCR reaction, so that the increase in emitted fluorescence...
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  3. Research: Sir3 mediates long-range chromosome interactions in budding yeast
    • Myriam Ruault,
    • Vittore F. Scolari,
    • Luciana Lazar-Stefanita,
    • Antoine Hocher,
    • Isabelle Loïodice,
    • Romain Koszul,
    • and Angela Taddei
    Genome Res. March 2021 31: 411-425; Published in Advance February 12, 2021, doi:10.1101/gr.267872.120
    ...occupancy along the rDNA locus probed by ChIP-qPCR using an anti-Sir3 antibody (Ruault et al. 2011) in WT (yAT232), GAL1p-SIR3 (yAT208), GAL1p-sir3-A2Q (yAT1205), and GAL1p-SIR3 sir2Δ (yAT772) strains. Primer pair 1 amplifies a region of the RDN25 locus, primer pair 2 amplifies a region in NTS1 (Non...
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