Searching journal content for articles similar to Nuovo et al. 4 (2): 89.

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  1. ARTICLE: In situ PCR: protocols and applications.
    • G J Nuovo
    Genome Res. February 1995 4: S151-S167
    ...Wax beads (Perkin-Elmer), increases the sensitivity of solution-phase PCR by at least two orders of magnitude. (6-8) We examined the contribution of the nonspecific DNA synthesis pathways during in situ PCR. (s'12) A strong primer-independent signal was present dur- ing in situ PCR. This initial observation...
  2. ARTICLE: Importance of different variables for enhancing in situ detection of PCR-amplified DNA.
    • G J Nuovo,
    • F Gallery,
    • R Hom,
    • P MacConnell,
    • and W Bloch
    Genome Res. May 1993 2: 305-312; doi:10.1101/gr.2.4.305
    ...be a necessary step with in situ PCR when cross-linking fixatives are used. Both solution phase and in situ PCR suffer from several side reactions that compete with target-specific amplifica- tion. Nonspecific DNA synthesis can fol- low extension of primers annealed to nontarget sequences ("mispriming...
  3. Methods: Nested Patch PCR enables highly multiplexed mutation discovery in candidate genes
    • Katherine Elena Varley and
    • Robi David Mitra
    Genome Res. November 2008 18: 1844-1850; Published in Advance October 10, 2008, doi:10.1101/gr.078204.108
    ..., a novel method for highly multiplexed PCR that is very specific, can sensitively detect SNPs and mutations, and is easy to implement. This is the first method that couples multiplex PCR with sample-specific DNA barcodes and next-generation sequencing to enable highly multiplex mutation discovery...
  4. METHODS: SNP Genotyping by Multiplexed Solid-Phase Amplification and Fluorescent Minisequencing
    • Michael H. Shapero,
    • Kerstin K. Leuther,
    • Anhthu Nguyen,
    • Melissa Scott,
    • and Keith W. Jones
    Genome Res. November 1, 2001 11: 1926-1934; Published in Advance October 15, 2001, doi:10.1101/gr.205001
    ...or spots were pre-cycled to decrease nonspecific amplification (94°C for 10 min, then 94°C for 45 sec, 45°C for 1 min, 72°C for 1 min for 15 cycles) in 1× GeneAmp PCR buffer (10 mM Tris-HCl, pH 8.3, 50 mM KCl, 1.5 mM MgCl2, 0.001% [w/v] gelatin) containing 0.25 ng/μL Escherichia coli genomic DNA (Sigma...
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