Searching journal content for articles similar to Nudelman et al. 28 (9): 1415.

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  1. ...al. 2019). Long-read RNA sequencing has refined existing gene annotations as well as characterized pervasive alternative splicing among organs (Abdel-Ghany et al. 2016; Wang et al. 2016a; Kuo et al. 2017; Li et al. 2018; Zhang et al. 2019).Although transcriptome complexity has been increasingly...
  2. ...between them. Although this suggests that R2C2 captures the transcriptome in a quantitative manner, it is not the focus of this analysis as the strength of long-read sequencing like R2C2 is not gene-level but instead isoform-level transcriptome analysis.Isoform identification and evaluationTo identify...
  3. ...overall contiguity metrics to other long-read assembled teleost s with a scaffold number and N50 length of 68–102 and 30–32 Mb, respectively (Table 1). All 24 chromosomes were assembled with a 0.4%–0.8% range of unassigned sequences. We found few ordering discrepancies and show significant chromosome...
  4. ...–TE transcripts is technically challenging, but long-read transcriptome sequencing will ease many of these difficulties and is therefore a promising avenue for future studies.Using bulk and single-cell RNA-seq to untangle temporal and lineage-specific patterns of TE expression, we observe broadly distinct...
  5. ...analysis of zebrafish TEs in the context of gene regulation is lacking. Here, we systematically investigate the epigenomic and transcriptomic landscape of TEs across 11 adult zebrafish tissues using multidimensional sequencing data. We find that TEs contribute substantially to a diverse array of regulatory...
  6. ...Table S4). To examine their quality, all annotations were compared to those of RefSeq, GENCODE (manual), GENCODE (automatic), Pacific Biosciences (PacBio) long read assembly, and MiTranscriptome in terms of the number of full-length independent transcripts. Although BIGTranscriptome reconstructed fewer...
  7. ...(Metzker 2010) have had automatic gene annotation applied to them, whereas high-quality finished s such as the human (InternationalHumanGenomeSequencingConsortium2004), mouse (Church et al. 2009), and zebrafish (Becker and Rinkwitz 2011) have manual annotation projects associated with them. Publicly...
  8. ...cells composed of nine 1-cell (zygote), 10midstage 2-cell, and five 4-cell embryos (Fig. 1A). The generation of data from every sister blastomere turned out indispensable for testing the hypotheses above.We deeply sequenced each library to produce on average 37.9 million 100-base long reads (Fig. 1B...
  9. ...in previous annotations owing to the splitting strategy. Finally, by integrating short-read and long-read transcriptomic data, we annotated 5′ UTR for 14,979 genes and 3′ UTR for 15,760 genes.By utilizing our assembly and annotation to reanalyze previous single-cell data, we found a substantial increase...
  10. ...-quality sequence and a variety of genetic tools are available for this model organism. However, existing annotation is still rudimentary, as it was mainly based on computational prediction and short-read RNA-seq data. Here we report a dynamic transcriptome landscape of medaka embryogenesis profiled by long-read...
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