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Methods: Large-scale screening for novel low-affinity extracellular protein interactions
- K. Mark Bushell,
- Christian Söllner,
- Benjamin Schuster-Boeckler,
- Alex Bateman,
- and Gavin J. Wright
Genome Res. April 2008 18: 622-630; Published in Advance February 22, 2008, doi:10.1101/gr.7187808
...-throughput interaction assays, with the yeast-two-hybrid (Y2H) and biochemical purification “TAP-tagging” techniques being especially successful. Due to the heterogeneity of protein–protein interactions and the practical limitations of large-scale screening, each assay must inevitably accept a certain rate of false...
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METHODS: Protein–Protein Interaction Panel Using Mouse Full-Length cDNAs
- Harukazu Suzuki,
- Yoshifumi Fukunishi,
- Ikuko Kagawa,
- Rintaro Saito,
- Hiroshi Oda,
- Toshinori Endo,
- Shinji Kondo,
- Hidemasa Bono,
- Yasushi Okazaki,
- and Yoshihide Hayashizaki
Genome Res. October 1, 2001 11: 1758-1765; Published in Advance September 20, 2001, doi:10.1101/gr.180101
...and predicted functions of a few uncharacterized proteins. In combination with our comprehensive mouse full-length cDNA clone bank covering a large part of the whole genes, our high-throughput assay system will discover many interactions to facilitate understanding of the function of uncharacterized proteins...
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Research: SWATH-MS-based proteogenomic analysis reveals the involvement of alternative splicing in poplar upon lead stress
- Fu-Yuan Zhu,
- Xin Chen,
- Yu-Chen Song,
- Lydia Pui Ying Lam,
- Yuki Tobimatsu,
- Bei Gao,
- Mo-Xian Chen,
- and Fu-Liang Cao
Genome Res. March 2023 33: 371-385; Published in Advance March 24, 2023, doi:10.1101/gr.277473.122
...by yeast two-hybrid (Y2H), but no interaction was observed (Supplemental Fig. S8B), indicating that each of these proteins can resist Pb without relying on mutual interaction. Furthermore, a significant decline in the Pb(II) contents was observed in both the PtHSP70AS1- and PtHSP70AS2-overexpressing lines...
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RECOMB Special/Review: Protein networks in disease
- Trey Ideker and
- Roded Sharan
Genome Res. April 2008 18: 644-652; doi:10.1101/gr.071852.107
....J. , Iyer, V.R. ( 2007 ) Genetic reconstruction of a functional transcriptional regulatory network . Nat. Genet. 39 : 683 – 687 . ↵ Huang, H. , Jedynak, B.M. , Bader, J.S. ( 2007 ) Where have all the interactions gone? Estimating the coverage of two-hybrid protein interaction maps . PLoS Comput. Biol. 3 : e...
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LETTER: New genes with roles in the C. elegans embryo revealed using RNAi of ovary-enriched ORFeome clones
- Anita G. Fernandez,
- Kristin C. Gunsalus,
- Jerry Huang,
- Ling-Shiang Chuang,
- Nancy Ying,
- Hsiao-lan Liang,
- Caroline Tang,
- Aaron J. Schetter,
- Charles Zegar,
- Jean-François Rual,
- David E. Hill,
- Valerie Reinke,
- Marc Vidal,
- and Fabio Piano
Genome Res. February 2005 15: 250-259; doi:10.1101/gr.3194805
...to characterize gene function on multiple levels, including gene expression patterns ( Spellman et al. 1998 ; Reinke et al. 2000 , 2004 ; Jiang et al. 2001 ; Arbeitman et al. 2002 ; Baugh et al. 2003 ), protein-protein interactions ( Uetz et al. 2000 ; Walhout et al. 2000 ; Ito et al. 2001 ; Giot et al. 2003 ; Li...

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