Searching journal content for articles similar to Nakane et al. 11 (3): 441.

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  1. LETTER: ACAPELLA-1K, A Capillary-Based Submicroliter Automated Fluid Handling System for Genome Analysis
    • Deirdre R. Meldrum,
    • Harold T. Evensen,
    • William H. Pence,
    • Stephen E. Moody,
    • David L. Cunningham,
    • and Peter J. Wiktor
    Genome Res. January 1, 2000 10: 95-104; doi:10.1101/gr.10.1.95
    ...the amount of disposables used to perform DNA sequencing. Compared with microplates, capillaries have a higher heat exchange rate for faster thermal cycling and may be handled both sequentially and in parallel. Capillaries are also natural pipettes, ideally suited for removing fluids from microplates...
  2. Methods: Simple, robust methods for high-throughput nanoliter-scale DNA sequencing
    • Duane E. Smailus,
    • Andre Marziali,
    • Philip Dextras,
    • Marco A. Marra,
    • and Robert A. Holt
    Genome Res. October 2005 15: 1447-1450; Published in Advance September 16, 2005, doi:10.1101/gr.4221805
    ...to 10 thermal cycling reactions, and their cost is offset from the savings achieved by reduced volume of sequence reaction mix. Sealed plates were thermocycled using Tetrad peltier thermal cyclers (MJ Research). Thermal cycling conditions for the reaction format presented here were 50 × (96°C, 10 sec...
  3. REVIEW: Automation for Genomics, Part One: Preparation for Sequencing
    • Deirdre Meldrum
    Genome Res. August 1, 2000 10: 1081-1092; doi:10.1101/gr.101400
    ...parallel thermal cycler for 96-, 192-, or 384-well microplates for up to 6144 samples/run. The thermocycler can run four sets of indepedendent heating and cooling protocols. Andre Marziali at the University of British Columbia (Vancouver, BC, Canada), in collaboration with the Stanford DNA Sequencing...
  4. REVIEW: Automation for Genomics, Part Two: Sequencers, Microarrays, and Future Trends
    • Deirdre Meldrum
    Genome Res. September 1, 2000 10: 1288-1303; doi:10.1101/gr.157400
    ...by laser fluorescence. (Y. Hayashizaki and G. Chapman, pers. comm., Sasaki et al. 1998 ). Online DNA Sequencers A couple of online systems have been developed to automate and integrate thermal cycling, purification, loading, and capillary electrophoresis steps. Samples are placed in the loop...
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