Searching journal content for articles similar to Merrihew et al. 18 (10): 1660.

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  1. ..., a Moerman Gene Knockout Laboratory subculture of N2 obtained from the Caenorhabditis Genetics Center (https://cgc.umn.edu) in 2002 and first described by Flibotte et al. (2010).C. elegans and gene annotation versionsIn analyzing gDNA from the N2 assembly, we have used the most recent stable version from...
  2. ...).Weuseda strand-specific strategy during RNA-seq library construction, and the accuracy of the strand separationwas confirmed to be higher than 99.8% (Methods). Hence, we could determine the transcript orientation with high confidence in the absence of gene annotations. RNA editing was found in both...
  3. ...relationships. It allowed us to classify and eliminate ambiguities inherently associated with any shotgun proteomics data set, to report a conservative list of protein identifications, and to seamlessly integrate data from previous transcriptomics studies. Manual validation of proteins unambiguously identified...
  4. ...Base (WS170) and confirm additional details of processing. For example, the total number of confirmed splice junctions was raised from 70,911 to over 98,000. The data also suggest thousands of modifications to WormBase annotations and identify new spliced junctions and genes not part of any Worm...
  5. ...( Fig.1B ). A second example is seen in Figure 2 , where the presence of two ecores in a region without annotation in the two insect s revealed the existence of a totally new gene, confirmed by a spliced mosquito cDNA. View larger version: In this window In a new window Figure 1 Exofish analysis...
  6. ...of Protein Expression of C. elegans Table 1 summarizes the results of protein expression for 118 plates of C. elegans ORFs. To achieve proteome-scale analysis, expression and solubility data were obtained using the same conditions for all genes. From the 10,167 unique ORFs, small-scale expression in E. coli...
  7. ...In these experiments we used TWINSCAN gene prediction followed by RT–PCR and direct sequencing to confirm partial gene structures in the newly sequenced rat . All 444 of the targets had significant similarity to known human genes, but they were at least partially missed by the April 2003 Ensembl annotation of the rat...
  8. ...species complements the current annotation strategies by identifying genes and improving gene structure. A total of 595,321 Brassica oleracea shotgun reads were sequenced by TIGR (The Institute for Genome Research) and the collaboration of Washington University and Cold Spring Harbor. Vicogenta (a...
  9. ...). When this procedure was tested on a set of elegans genes for which the WormBase annotation was known to be correct, the correct model was chosen 92% of the time. This number only bears on the sets of overlapping gene models that include at least one correct model. It provides no information about...
  10. ...with the structural analysis of the RNA genes that regulate them. Proteomics With the well-annotated C. elegans in hand, both the study of individual proteins and the study of interactions among those proteins can proceed. In a high-throughput proteomic effort to confirm protein-coding genes, G. Merrihew, J.H. Thomas...
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