Searching journal content for articles similar to Martinez et al. 6 (1): 58.

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  1. Method: ABCA4 midigenes reveal the full splice spectrum of all reported noncanonical splice site variants in Stargardt disease
    • Riccardo Sangermano,
    • Mubeen Khan,
    • Stéphanie S. Cornelis,
    • Valerie Richelle,
    • Silvia Albert,
    • Alejandro Garanto,
    • Duaa Elmelik,
    • Raheel Qamar,
    • Dorien Lugtenberg,
    • L. Ingeborgh van den Born,
    • Rob W.J. Collin,
    • and Frans P.M. Cremers
    Genome Res. January 2018 28: 100-110; Published in Advance November 21, 2017, doi:10.1101/gr.226621.117
    ...DNA. PCR conditions were a first denaturation step of 94°C for 5 min, followed by 35 cycles of melting (94°C for 30 sec each), annealing (58°C for 30 sec), and extension (72°C for 5 min) steps, with a final elongation step of 72°C for 2 min. The RT-PCR products were run on a 2% (w/v) agarose gel...
  2. Method: RNA sequencing of Sleeping Beauty transposon-induced tumors detects transposon-RNA fusions in forward genetic cancer screens
    • Nuri A. Temiz,
    • Branden S. Moriarity,
    • Natalie K. Wolf,
    • Jesse D. Riordan,
    • Adam J. Dupuy,
    • David A. Largaespada,
    • and Aaron L. Sarver
    Genome Res. January 2016 26: 119-129; Published in Advance November 9, 2015, doi:10.1101/gr.188649.114
    ...and include fusions to Rb1,Wrn,Notch2, and Csf1r. These results indicate that a significant number of transposon insertions are being missed by LM-PCR basedmethodology but can be inferred by the presence of RNA fusions. RT-PCR was used to validate that transposon-derived fusion sequences were present...
  3. Research: Evolutionary dynamism of the primate LRRC37 gene family
    • Giuliana Giannuzzi,
    • Priscillia Siswara,
    • Maika Malig,
    • Tomas Marques-Bonet,
    • NISC Comparative Sequencing Program,
    • James C. Mullikin,
    • Mario Ventura,
    • and Evan E. Eichler
    Genome Res. January 2013 23: 46-59; Published in Advance October 11, 2012, doi:10.1101/gr.138842.112
    ...to cerebellumwhere this drops to 26%. Inmouse testis, splice form 1a predominates with very little alternative splicing observed as suggested by the RT-PCR profile on agarose gel (Fig. 6B). Human LRRC37A4 transcripts are unique—the gene structure lacks exons 6 and 7 and has a different splice acceptor site within...
  4. RESEARCH: Homologs of the Yeast Longevity Gene LAG1 in Caenorhabditis elegans and Human
    • James C. Jiang,
    • Paul A. Kirchman,
    • Marek Zagulski,
    • Jay Hunt,
    • and S. Michal Jazwinski
    Genome Res. December 1, 1998 8: 1259-1272; doi:10.1101/gr.8.12.1259
    ...P]ATP and reverse transcription was performed as for RT–PCR, except specific reverse primers downstream from the transcription start site were used instead of oligo(dT) and random primers. The extended, single-stranded DNA was subjected to electrophoresis on a sequencing gel alongside the sequencing...
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