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Marra et al. 6 (11): 1118
.
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Research
:
Frequent somatic transfer of mitochondrial DNA into the nuclear genome of human cancer cells
Young Seok Ju
,
Jose M.C. Tubio
,
William Mifsud
,
Beiyuan Fu
,
Helen R. Davies
,
Manasa Ramakrishna
,
Yilong Li
,
Lucy Yates
,
Gunes Gundem
,
Patrick S. Tarpey
,
Sam Behjati
,
Elli Papaemmanuil
,
Sancha Martin
,
Anthony Fullam
,
Moritz Gerstung
,
ICGC Prostate Cancer Working Group
,
ICGC Bone Cancer Working Group
,
ICGC Breast Cancer Working Group
,
Jyoti Nangalia
,
Anthony R. Green
,
Carlos Caldas
,
Åke Borg
,
Andrew Tutt
,
Ming Ta Michael Lee
,
Laura J. van't Veer
,
Benita K.T. Tan
,
Samuel Aparicio
,
Paul N. Span
,
John W.M. Martens
,
Stian Knappskog
,
Anne Vincent-Salomon
,
Anne-Lise Børresen-Dale
,
Jórunn Erla Eyfjörd
,
Ola Myklebost
,
Adrienne M. Flanagan
,
Christopher Foster
,
David E. Neal
,
Colin Cooper
,
Rosalind Eeles
,
G. Steven Bova
,
Sunil R. Lakhani
,
Christine Desmedt
,
Gilles Thomas
,
Andrea L. Richardson
,
Colin A. Purdie
,
Alastair M. Thompson
,
Ultan McDermott
,
Fengtang Yang
,
Serena Nik-Zainal
,
Peter J. Campbell
,
and
Michael R. Stratton
Genome Res.
June 2015
25
:
814
-
824
;
Published in Advance
May 11, 2015
,
doi:
10.1101/gr.190470.115
...ancestral cancer
clone
or in normal somatic cells prior to neoplastic change. Nuclear
transfer
of mtDNA is unexpectedly frequent in human somatic cells To obtain a perspective on the frequency ofmitochondrial-nuclear DNA translocation, we compared its rate to that of intranuclear 0 20 40 60 80 10 240 100...
Abstract
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Supplemental Material
OPEN ACCESS ARTICLE
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LETTER
:
High Throughput Fingerprint Analysis of Large-Insert Clones
Marco A. Marra
,
Tamara A. Kucaba
,
Nicole L. Dietrich
,
Eric D. Green
,
Buddy Brownstein
,
Richard K. Wilson
,
Ken M. McDonald
,
LaDeana W. Hillier
,
John D. McPherson
,
and
Robert H. Waterston
Genome Res.
November 1, 1997
7
:
1072
-
1084
;
doi:
10.1101/gr.7.11.1072
...High Throughput Fingerprint Analysis of Large-
Insert
Clones
Marco A. Marra 1 , 5 , Tamara A. Kucaba 1 , Nicole L. Dietrich 1 , Eric D. Green 2 , Buddy Brownstein 4 , Richard K. Wilson 1 , 3 , Ken M. McDonald 1 , 3...
Abstract
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ARTICLE
:
Mapping the human Y chromosome by fingerprinting cosmid clones.
K Taylor
,
N Hornigold
,
D Conway
,
D Williams
,
Z Ulinowski
,
M Agochiya
,
P Fattorini
,
P de Jong
,
P F Little
,
and
J Wolfe
Genome Res.
April 1996
6
:
235
-
248
;
doi:
10.1101/gr.6.4.235
...Mapping the human Y chromosome by fingerprinting cosmid
clones
. K Taylor , N Hornigold , D Conway , D Williams , Z Ulinowski , M Agochiya , P Fattorini , P de Jong , P F Little , and J Wolfe...
Abstract
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References
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RESEARCH
:
Analysis of the 1.1-Mb Human α/δ T-Cell Receptor Locus with Bacterial Artificial Chromosome Clones
Cecilie Boysen
,
Melvin I. Simon
,
and
Leroy Hood
Genome Res.
April 1, 1997
7
:
330
-
338
;
doi:
10.1101/gr.7.4.330
...with BAC
clones
. Seventeen BAC
clones
covered the 1.1-Mb α/δ locus, with the exception of one small gap that was expected from the coverage that a 3.7-fold BAC library is likely to provide. The end sequences of the BAC
inserts
could be obtained directly from the BAC DNA by sequencing with the chain...
Abstract
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ARTICLE
:
Chromosomal localization and expressed sequence tag generation of clones from a normalized human adult thymus cDNA library.
J E Lamerdin
,
R S Athwal
,
M S Kansara
,
A K Sandhu
,
S R Patanjali
,
S M Weissman
,
and
A V Carrano
Genome Res.
November 1995
5
:
359
-
367
;
doi:
10.1101/gr.5.4.359
...sequence tags) from a directionally
cloned
human infant brain cDNA library. Nature Genet. 4: 373 – 380. Athwal, R.S., Smarsh, M. Searle, B.M. and Deo. S.S. 1985. Integration of a dominant selectable marker into human chromosomes and
transfer
of marked chromosomes to mouse cells by microcell fusion. Somat...
Abstract
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References
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ARTICLES
:
Use of a fluorescent-PCR reaction to detect genomic sequence copy number and transcriptional abundance.
P W Chiang
,
W J Song
,
K Y Wu
,
J R Korenberg
,
E J Fogel
,
M L Van Keuren
,
D Lashkari
,
and
D M Kurnit
Genome Res.
October 1996
6
:
1013
-
1026
;
doi:
10.1101/gr.6.10.1013
.... No PCR
Energy
transfer
-~
fluorescence
+ the ethidium bromide-gel-based assay by at least fourfold. F primer 1 primer 2 PCR No
energy
transfer
--~
fluorescence
- primer 2 F primer 1 Figure 2 Schematic representation of
energy
transfer
of the Ampli- Sensor in PCR. Primer 2 is the excess primer in the first...
Abstract
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Research
:
Nanopore direct RNA sequencing reveals METTL2A-mediated m
3
C sites in poly(A) RNA
Shuhei Mitsutomi
,
Anzu Sugawara
,
Masahide Seki
,
Yutaka Suzuki
,
Sotaro Miyao
,
Haozhe Du
,
Kenji Takahashi
,
Yusuke Mizukami
,
Kenzui Taniue
,
and
Nobuyoshi Akimitsu
Genome Res.
November 2025
35
:
2406
-
2417
;
Published in Advance
October 30, 2025
,
doi:
10.1101/gr.280269.124
...% FBS and 1 mM sodium pyruvate.Molecular
cloning
The construct for expressing FLAG-tagged METTL2A (pcDNA3.1-FLAG-METTL2A) was generated by amplifying the open reading frame (ORF) of METTL2A from AsPC-1 cell cDNA using PrimeSTAR GXL DNA Polymerase (Takara, #R050A) and
inserting
it into the EcoRV and Xho...
Abstract
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RESOURCE
:
Bacterial Artificial Chromosome Libraries for Mouse Sequencing and Functional Analysis
Kazutoyo Osoegawa
,
Minako Tateno
,
Peng Yeong Woon
,
Eirik Frengen
,
Aaron G. Mammoser
,
Joseph J. Catanese
,
Yoshihide Hayashizaki
,
and
Pieter J. de Jong
Genome Res.
January 1, 2000
10
:
116
-
128
;
doi:
10.1101/gr.10.1.116
...functional gene studies. For genome mapping and sequencing, we prepared BAC libraries from the 129S6/SvEvTac and the C57BL/6J strains. The average
insert
sizes for the three libraries range between 130 kb and 200 kb. Based on the numbers of
clones
and the observed average
insert
sizes, we estimate each...
Abstract
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METHODS
:
Evaluation of Single Nucleotide Polymorphism Typing with Invader on PCR Amplicons and Its Automation
Charles A. Mein
,
Bryan J. Barratt
,
Michael G. Dunn
,
Thorsten Siegmund
,
Annabel N. Smith
,
Laura Esposito
,
Sarah Nutland
,
Helen E. Stevens
,
Amanda J. Wilson
,
Michael S. Phillips
,
Nancy Jarvis
,
Scott Law
,
Monika de Arruda
,
and
John A. Todd
Genome Res.
March 1, 2000
10
:
330
-
343
;
doi:
10.1101/gr.10.3.330
...in a genotyping laboratory and tested its automation. The Invader technology uses a Flap Endonuclease for allele discrimination and a universal
fluorescence
resonance
energy
transfer
(FRET) reporter system. Three hundred and eighty-four individuals were genotyped across a panel of 36 SNPs and one
insertion
...
Abstract
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Select this article
Method
:
Transcript amplification from single bacterium for transcriptome analysis
Yun Kang
,
Michael H. Norris
,
Jan Zarzycki-Siek
,
William C. Nierman
,
Stuart P. Donachie
,
and
Tung T. Hoang
Genome Res.
June 2011
21
:
925
-
935
;
Published in Advance
May 2, 2011
,
doi:
10.1101/gr.116103.110
...) Bright-field mode showing the section of the membrane where the single bacterium had been. (E )
Fluorescence
mode confirming that the bacterium of interest has been
transferred
from the membrane slide to the PCR tube lid. 926 Genome Research www..org Kang et al. B. thailandensis cellswere isolated (Fig...
Abstract
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