Searching journal content for articles similar to Marra et al. 6 (11): 1118.

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  1. ...ancestral cancer clone or in normal somatic cells prior to neoplastic change. Nuclear transfer of mtDNA is unexpectedly frequent in human somatic cells To obtain a perspective on the frequency ofmitochondrial-nuclear DNA translocation, we compared its rate to that of intranuclear 0 20 40 60 80 10 240 100...
  2. ...High Throughput Fingerprint Analysis of Large-InsertClones Marco A. Marra 1 , 5 , Tamara A. Kucaba 1 , Nicole L. Dietrich 1 , Eric D. Green 2 , Buddy Brownstein 4 , Richard K. Wilson 1 , 3 , Ken M. McDonald 1 , 3...
  3. ...Mapping the human Y chromosome by fingerprinting cosmid clones. K Taylor , N Hornigold , D Conway , D Williams , Z Ulinowski , M Agochiya , P Fattorini , P de Jong , P F Little , and J Wolfe...
  4. ...with BAC clones. Seventeen BAC clones covered the 1.1-Mb α/δ locus, with the exception of one small gap that was expected from the coverage that a 3.7-fold BAC library is likely to provide. The end sequences of the BAC inserts could be obtained directly from the BAC DNA by sequencing with the chain...
  5. ...sequence tags) from a directionally cloned human infant brain cDNA library. Nature Genet. 4: 373 – 380. Athwal, R.S., Smarsh, M. Searle, B.M. and Deo. S.S. 1985. Integration of a dominant selectable marker into human chromosomes and transfer of marked chromosomes to mouse cells by microcell fusion. Somat...
  6. .... No PCR Energy transfer-~ fluorescence + the ethidium bromide-gel-based assay by at least fourfold. F primer 1 primer 2 PCR No energy transfer--~ fluorescence - primer 2 F primer 1 Figure 2 Schematic representation of energy transfer of the Ampli- Sensor in PCR. Primer 2 is the excess primer in the first...
  7. ...% FBS and 1 mM sodium pyruvate.Molecular cloningThe construct for expressing FLAG-tagged METTL2A (pcDNA3.1-FLAG-METTL2A) was generated by amplifying the open reading frame (ORF) of METTL2A from AsPC-1 cell cDNA using PrimeSTAR GXL DNA Polymerase (Takara, #R050A) and inserting it into the EcoRV and Xho...
  8. ...functional gene studies. For genome mapping and sequencing, we prepared BAC libraries from the 129S6/SvEvTac and the C57BL/6J strains. The average insert sizes for the three libraries range between 130 kb and 200 kb. Based on the numbers of clones and the observed average insert sizes, we estimate each...
  9. ...in a genotyping laboratory and tested its automation. The Invader technology uses a Flap Endonuclease for allele discrimination and a universal fluorescence resonance energy transfer (FRET) reporter system. Three hundred and eighty-four individuals were genotyped across a panel of 36 SNPs and one insertion...
  10. ...) Bright-field mode showing the section of the membrane where the single bacterium had been. (E ) Fluorescence mode confirming that the bacterium of interest has been transferred from the membrane slide to the PCR tube lid. 926 Genome Research www..org Kang et al. B. thailandensis cellswere isolated (Fig...
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