Searching journal content for articles similar to Luan et al. 14 (10b): 2102.

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  1. ...a completely defined reference and corresponding isogenic wild-type strain for C. elegans, allowing unique opportunities for model and systems biology.The nematode Caenorhabditis elegans is a model for biology ranging from mechanistic functions of individual proteins and RNAs to multicellular interactions...
  2. ...targeted mRNAs and mitigate truncated protein production. The presence of independent degradation pathways conflicts with genetic evidence supporting the requirement of all of SMG-1 through SMG-7 in Caenorhabditis elegans’ NMD (Hodgkin et al. 1989; Cali et al. 1999; Anders et al. 2003).A contemporary SMG-5...
  3. ...targeted SMC-3 subunit for auxin-inducible degradation. WAPL-1 is encoded by wapl-1 in C. elegans (Gandhi et al. 2006; Kueng et al. 2006). The two proteins SMC-3 and WAPL-1 were C-terminally tagged with degron-GFP at the endogenous locus for auxin-inducible depletion, as employed previously (Zhang et al...
  4. ...organism Encyclopedia of Regulatory Networks) consortia to systematically assay TF binding events in vivo in two major model organisms, Drosophila melanogaster (fly) and Caenorhabditis elegans (worm). These data sets comprise 605 TFs identifying 3.6 M sites in the fly and 356 TFs identifying 0.9 M sites...
  5. ...and Ruvkun 2020).Endogenous siRNAs may constitute a distinct class of small RNAs largely overlooked in C. elegans. Presumably, such siRNAs are rare in wild-type C. elegans since they have not emerged from high-throughput small RNA sequencing (sRNA-seq) studies characterizing small RNAs (Ruby et al. 2006...
  6. .... We recently addressed this limitation by developing a protocol to isolate undifferentiated germ nuclei (IGN) at sufficient numbers that allow for genomic assays (Han et al. 2019). Over the past few years, we and others have successfully used this protocol for different high-throughput sequencing...
  7. ...previous studies, which tend to focus srWGS applications to SNVs, short insertions, and deletions (Maroilley and Tarailo-Graovac 2019). With such an approach, whole- sequencing based on short reads constitutes an interesting way to explore, in a high-throughput manner, the entire spectrum of genomic...
  8. ...variants in VC2010, 14 (74%) were duplications that have the potential to encode near-identical paralogs of C. elegans genes (Fig. 3G; Supplemental Table S18).New genes in C. elegansTo find possible new genes in the VC2010 assembly, we predicted 21,070 protein-coding genes in the VC2010 assembly...
  9. ...accessibility profiles of single cells has improved greatly. This technology relies on the assay for transposase-accessible chromatin followed by high-throughput sequencing (ATAC-seq) (Buenrostro et al. 2013), which treats permeabilized nuclei with a hyperactive Tn5 transposome from prokaryotes (Adey et al...
  10. .... elegans that parallels the evolutionary forces and constraints observed across metazoa.Alternative precursor mRNA (pre-mRNA) splicing is a critical layer of gene expression in multicellular animals and plays a key role in increasing the functional diversity of proteins within an organism. Indeed...
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