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  1. .... These processes are fundamental for inheritance but incompletely understood. We analyzed these processes in the frog Xenopus laevis, whose sperm can be assembled into functional pronuclei in egg extracts in vitro. In such extracts, cohesin extrudes DNA into loops, but in vivo cohesin only assembles topologically...
  2. .... 2011. Scaffolding pre-assembled contigs using SSPACE. Bioinformatics 27: 578–579. Bowers JR, Kitchel B, Driebe EM,MacCannell DR, Roe C, Lemmer D, deMan T, Rasheed JK, Engelthaler DM, Keim P, et al. 2015. Genomic analysis of the emergence and rapid global dissemination of the clonal group 258 Klebsiella...
  3. ...Recent independent emergence of multiple multidrug-resistant Serratia marcescens clones within the United Kingdom and Ireland Danesh Moradigaravand 1 , Christine J. Boinett 1 , Veronique Martin 2 , Sharon J. Peacock 1 , 3 , 4 and Julian...
  4. ...SiCloneFit: Bayesian inference of population structure, genotype, and phylogeny of tumor clones from single-cell sequencing data Hamim Zafar1,2, Nicholas Navin3, Ken Chen2 and Luay Nakhleh1 1Department of Computer Science, Rice University, Houston, Texas 77005, USA; 2Department of Bioinformatics...
  5. ..., Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom Abstract Contigs have been assembled, and over 2800 clones selected for sequencing for human chromosomes 9, 10 and 13. Using the FPC (FingerPrinted Contig) software, the contigs are assembled with markers and complete...
  6. ...by polymerase chain reaction (PCR) amplification of transcripts in 96-well plates using gene-specific primer pairs flanking the ORFs. Amplicons are cloned into a sequencing vector, and full-length sequences are obtained. Sequences are processed and assembled using Phred and Phrap , and analyzed using Consed...
  7. ...genomic sequence onto chromosomes (for selected BAC clones, see Supplemental Table S1). We selected 139 BAC clones to cover 100 of the largest scaffolds assembled in the Ciona draft (v. 1.0) (Scaffold_1 to Scaffold_100), and another set of 23 BAC clones was selected to increase the accuracy...
  8. ...cloning procedure. DNA sequencing reveals high-fidelity capture of 87% of known and predicted yeast ORFs To provide a well-annotated collection, we sequenced the complete insert (including the 5′ and 3′ att B sequences), assembled sequence contigs, and compared the assemblies to corresponding reference...
  9. ...assembly was conducted using a tolerance of 3 (see Methods: Computer Analysis and Contig Construction) all 107 rDNA clones that had been identified manually were placed into a single contig by FPC. One additional clone (G31K19) was also identified and placed in this contig by FPC. Comparison of this clone’s...
  10. ...-to-telomere scaffolds by twofold, reduce runtime by fourfold, and improve assembly correctness. On a panel of 19 human s, Verkko2 assembles an average of 39 of 46 complete chromosomes as scaffolds, with 21 of these assembled as gapless contigs. Together, these improvements enable telomere-to-telomere comparative...
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