Searching journal content for articles similar to Liu et al. 27 (10): 1685.

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  1. ...stress conditions (Geisberg et al. 2023). Moreover, the poly(A) sites from another yeast species Schizosaccharomyces pombe show different sequence composition versus S. cerevisiae (Liu et al. 2017). Currently, the polyadenylation complexes in S. pombe remain uncharacterized. It remains an open question...
  2. ...studies as well as new therapeutic angles to target eukaryotic pathogens.Cleavage and polyadenylation (CPA) are key steps in eukaryotic mRNA maturation, specifying the 3′ end of the transcript and the addition of the poly(A) tail. Required for the proper expression of nearly all mRNAs, the site of pre...
  3. ...we leverage Oxford Nanopore Technologies native RNA sequencing (dRNA), as well as ribosome profiling data, to uncover the full range of polyadenylated transcripts and translated open reading frames. We identify 332 alternative isoforms affecting the coding sequences of 262 different genes, 97...
  4. ..., Rutgers University, New Brunswick, New Jersey 08901, USA Corresponding author: btian@rutgers.eduAbstractCleavage and polyadenylation is essential for 3′ end processing of almost all eukaryotic mRNAs. Recent studies have shown widespread alternative cleavage and polyadenylation (APA) events leading to m...
  5. ..., in particular, the origin of AS. Saccharomyces cerevisiae and Schizosaccharomyces pombe are the two most documented unicellular model organisms. S. cerevisiae has ∼280 intron-containing genes, and the vast majority of them have a single intron. On the contrary, >2200 genes in S. pombe are currently known...
  6. ...RNP) complex involved in splicing and suppression of cryptic intronic polyadenylation similarly stimulates elongation and facilitates transcription of long genes (Mimoso and Adelman 2023). Furthermore, RNAPII elongation rates are modulated in response to certain stresses and stimuli such as heat shock and UV...
  7. ...). For reference, standard gene names for enriched RPs are given in Supplemental Fig. S2B. (B) Structure of the Saccharomyces cerevisiae 80S ribosome [PDB:5MC6] (Schmidt et al. 2016). RPs with S. pombe orthologs that were highly significantly enriched on poly(A)+ RNA are designated in blue. For reference, the m...
  8. ...at least 1 kb from any other genes on the same strand (S. pombe: n = 874; S. cerevisiae: n = 1101). We then generated scaled composite profiles in which the region between 300 bp downstream from the observed TSS and 300 bp upstream of the cleavage and polyadenylation signal (CPS) is scaled to the same...
  9. ...), indicating that a substantial fraction of partially spliced transcripts will be stable, polyadenylated, cytoplasmic mRNAs that likely represent alternative mRNA isoforms in S. pombe (Kuang et al. 2017). In conclusion, LRS of nascent transcripts provides a snapshot of splicing states for multi...
  10. ...Identification of programmed translational -1 frameshifting sites in the genome of Saccharomyces cerevisiae Michaël Bekaert 1 , Hugues Richard 2 , Bernard Prum 2 , and Jean-Pierre Rousset 1 , 3 1 Institut de Génétique et...
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