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  1. ...al. 2019). The other 70% of lowly ribosome-associated isoforms showed the opposite characteristic regarding 3′ UTR GC-content, which is concordant with reports that suggest an inverse relationship between 3′ UTR GC-content and mRNA stability (Litterman et al. 2019). Overall, broad measurements like...
  2. ...2000; Ezzeddine et al. 2007) and Rngtt is anmRNA 59-guanyltransferase capping enzyme (Pillutla et al. 1998). To our knowledge, this is the first report implicating modulation of mRNA processing and stability in metastasis. Analysis of expression data reveals modulation of network expression...
  3. ...observe after 4NQO treatment, especially if these RNAs are inherently unstable. Two types of yeast mRNAs fall in this category. First, transcripts with very long 39 UTRs are subject to destabilization through nonsense-mediated decay (NMD) (Nicholson et al. 2010; Parker 2012). In our data set, eight...
  4. ...–DNA sequence differences (RDDs) in the mRNA. We showed these sequence differences are propagated to proteins, as we identified peptides encoded by the RNA sequences in addition to those by the DNA sequences at RDD sites. RDDs are significantly enriched at regions with R-loops. A screen of yeast mutants showed...
  5. ...-normalized first-strand cDNA independently synthesized from the same preparation of mRNA. In order to increase specificity and sensitivity of the assay, we utilized nested primer strategy. Only clones positive in both assays were considered validated. PCR primers were successfully designed for 21 of the 24...
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