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Research: Dynamics and consequences of differential RNA isoform production during cardiomyocyte fate determination and early-stage maturation
- Joanna Delimata-Raczek,
- Natalia Koralewska,
- Bart Krist,
- Magdalena Rakoczy,
- Marek Figlerowicz,
- and Ireneusz Stolarek
Genome Res. April 3, 2026 ; Published in Advance April 3, 2026, doi:10.1101/gr.281150.125
..., overlooks a crucial layer of regulatory complexity: the expression of distinct transcript isoforms. Alternative transcription and splicing generate multiple mRNA variants from a single gene, enabling the production of functionally diverse proteins that fine-tune cellular processes. During development...
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Research: Saturation mutagenesis reveals manifold determinants of exon definition
- Shengdong Ke,
- Vincent Anquetil,
- Jorge Rojas Zamalloa,
- Alisha Maity,
- Anthony Yang,
- Mauricio A. Arias,
- Sergey Kalachikov,
- James J. Russo,
- Jingyue Ju,
- and Lawrence A. Chasin
Genome Res. January 2018 28: 11-24; Published in Advance December 14, 2017, doi:10.1101/gr.219683.116
...subunits U2AF65 (U2AF2) or U2AF35 (U2AF1) or to either of the U1 snRNP specific proteins U1A or U1–70K. The RNAs recovered from each of these four IPs were extracted and subjected to deep sequencing. Enrichment indices analogous to splicing EIs could be calculated for almost all of the mutant molecules...

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