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ARTICLES: A novel method for site-directed mutagenesis using PCR and uracil DNA glycosylase.
- A Rashtchian,
- C G Thornton,
- and G Heidecker
Genome Res. November 1992 2: 124-130; doi:10.1101/gr.2.2.124
.... 20. Lundberg, K.S., Shoemaker, D.D. Adams, M.W.W. Short, J.A. Sorge, J.A. and Mathur. E.J. 1991 . high-fidelity amplification using a thermostable DNA polymerase isolated from Pyrococcus furiosus . Gene 108 : 1 – 6 . A Novel Method for Site-directed Mutagenesis Using PCR and Uracil DNA Glycosylase...
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Method: Increasing frequencies of site-specific mutagenesis and gene targeting in Arabidopsis by manipulating DNA repair pathways
- Yiping Qi,
- Yong Zhang,
- Feng Zhang,
- Joshua A. Baller,
- Spencer C. Cleland,
- Yungil Ryu,
- Colby G. Starker,
- and Daniel F. Voytas
Genome Res. March 2013 23: 547-554; Published in Advance January 2, 2013, doi:10.1101/gr.145557.112
...screening for mutations by PCR. Interestingly, NHEJ mutagenesis and GT at the ADH1 locus were enhanced by sixfold to eightfold and threefold to fourfold, respectively, in a smc6b mutant. The increase in NHEJ-mediated mutagenesis by loss of SMC6B was further confirmed using ZFNs that target two other...
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ARTICLES: Randomization of genes by PCR mutagenesis.
- R C Cadwell and
- G F Joyce
Genome Res. August 1992 2: 28-33; doi:10.1101/gr.2.1.28
...by PCR mutagenesis. R C Cadwell and G F Joyce Department of Chemistry, Scripps Research Institute, La Jolla, California 92037. Abstract A modified polymerase chain reaction (PCR) was developed to introduce random point mutations into cloned genes. The modifications were made to decrease the fidelity...
ARTICLE: Unwanted mutations in PCR mutagenesis: avoiding the predictable.
- K D Smith,
- A Valenzuela,
- J L Vigna,
- K Aalbers,
- and C T Lutz
Genome Res. February 1993 2: 253-257; doi:10.1101/gr.2.3.253
...of amplif ication. Our frequency of errors falls wi th in this theoretical range, and, more importantly, the fidelity of Taq polymerase permits faithful replication of DNA used in PCR mutagenesis. We typically l imit the number of essential (i.e., amino acid coding) nucleotides to less than 300, thereby...
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ARTICLE: A highly efficient procedure for site-specific mutagenesis of full-length plasmids using Vent DNA polymerase.
- S Byrappa,
- D K Gavin,
- and K C Gupta
Genome Res. November 1995 5: 404-407; doi:10.1101/gr.5.4.404
.... 1992. A novel method for site- directed mutagenesis using PCR and uracil glycosylase. PCR Methods ApDlic. 2: 124-130. 5. Wong, F. and M. Komaromy. 1995. Site- directed mutagenesis u ing thermDstal~le enzymes. BioTechniques 18: 1034-1038. 6. Cariello, N.F., J.A. Swenberg, and T.R. Skopek. 1991. Fidelity...
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ARTICLE: Background-minimized cassette mutagenesis by PCR using cassette-specific selection markers: a useful general approach for studying structure-function relationships of multisubstrate enzymes.
- K Majumder,
- F A Fattah,
- A Selvapandiyan,
- and R K Bhatnagar
Genome Res. February 1995 4: 212-218
...be used to specifically remove/re- duce the wild-type, or the nonmutant, background clones. Even the PCR-based efficient multiple mutagenesis methods like splicing by overlap extension (SOE) by PCR, (7) stepwise elongation of se- quence (SES) by PCR, (8'0~ site-directed mono- and multiple mutagenesis...
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ARTICLE: Mutagenic oligonucleotide-directed PCR amplification (Mod-PCR): an efficient method for generating random base substitution mutations in a DNA sequence element.
- L W Chiang,
- I Kovari,
- and M M Howe
Genome Res. February 1993 2: 210-217; doi:10.1101/gr.2.3.210
...the low muta- genic activity of PCR itself (-0.08% per base in our hands for >25,000 bases se- quenced), and demonstrating the fidel- ity of restoration of WT sequences TABLE 1 Assessment of Degree of Randomness of Mutagenesis Method A. Frequency of substitution tonon-WT nucleotides Non-WT Number...
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REVIEW: Sequencing of PCR-amplified DNA.
- I S Bevan,
- R Rapley,
- and M R Walker
Genome Res. May 1992 1: 222-228; doi:10.1101/gr.1.4.222
...differences, in all situations the purity and concentration of PCR-amplified DNA template used remains the most critical factor determining the efficiency and reliability of nucleotide sequencing methods. Footnotes Copyright © Cold Spring Harbor Laboratory Press Bevan, I S Bevan I S Walker...
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GENOME METHODS: Fidelity and Mutational Spectrum of Pfu DNA Polymerase on a Human Mitochondrial DNA Sequence
- Paulo André,
- Andrea Kim,
- Konstantin Khrapko,
- and William G. Thilly
Genome Res. August 1, 1997 7: 843-852; doi:10.1101/gr.7.8.843
...doublings, a series of dilutions followed by PCR amplifications were performed. The efficiency of Pfu PCR under the conditions used is ∼66%. This is somewhat lower than can be achieved for other thermostable DNA polymerases, but the higher fidelity more than makes up for the need for a few extra cycles. We...
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ARTICLE: PCR induction of a TaqI restriction site at any CpG dinucleotide using two mismatched primers (CpG-PCR).
- S D O'Dell,
- S E Humphries,
- and I N Day
Genome Res. June 1996 6: 558-568; doi:10.1101/gr.6.6.558
....W. , Perry, T.R. , Silverman. L.M. Friedman, K.J., Highsmith, W.E. Prior, T.W. Perry, T.R. and Silverman. L.M. 1990 . Cystic fibrosis deletion mutation detected by PCR-mediated site-directed mutagenesis. Clin. Chem. 36 : 695 – 696 . Gaffney, B.L. , Jones. R.A. Gaffney, B.L. and Jones. R.A. 1989...

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