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  1. ...with a reference-guided assembly approach to generate an improved, high-quality assembly, TcasONT, of the model beetle Tribolium castaneum. Enriched by 45 Mb in repetitive regions, the new assembly comprises almost the entire sequence. We use the enhanced assembly to conduct global and in-depth analyses...
  2. ...DNAs are particularly useful.The flour beetle Tribolium freemani belongs to the genus that includes some of the most important pests of stored agricultural products (Sokoloff 1972). The economically important Tribolium species are also an excellent platform for the study of satellite DNAs, which occupy up to 60...
  3. ...expectations for when differential gene expression should matter. Here, we collated existing data into a gene-regulatory network (GRN) and performed developmental transcriptomics across different environmental conditions, genetic backgrounds, and mutants to assess the regulatory logic of mouth-form plasticity...
  4. ....279351.124 ↵Volarić M, Despot-Slade E, Veseljak D, Mravinac B, Meštrović N. 2024. Long-read assembly of the insect model organism Tribolium castaneum reveals spread of satellite DNA in gene-rich regions by recurrent burst events. Genome Res (this issue) 34: 1878–1894. doi:10.1101/gr.279225.124 ↵Zeglinski...
  5. ...enrichments were observed for single-tissue outliers but not multitissue outliers (Supplemental Fig. S5E).Watershed-SV integrates genomic annotations and transcriptomic signals to prioritize rare functional SVsThe co-occurrence of expression outliers with rare SVs provides the opportunity to jointly use...
  6. ...gastrulation by growth and cell division, and patterning occurs in a cellularized environment via an oscillatory mechanism (Sarrazin et al. 2012). Inmany respects, short germband embryogenesis therefore more closely resembles the segmentation of vertebrate embryos. The red flour beetleTribolium castaneum...
  7. ..., Pittsburgh, Pennsylvania 15213, USA Corresponding authors: jianma@cs.cmu.edu, skrieger@andrew.cmu.eduAbstractSpatial transcriptomics (ST) has transformed our understanding of tissue architecture and cellular interactions, but integrating ST data across platforms remains challenging due to differences in gene...
  8. ...the reference, such as disease- and trait-associated variants or engineered sequences. Recent work has applied synthetic regulatory genomics to characterized dozens of deletions, inversions, and rearrangements of DNase I hypersensitive sites (DHSs). Here, we use the state-of-the-art model Enformer to predict...
  9. ...spRefine denoises and imputes spatial1 transcriptomics with a reference-free framework2 powered by genomic language model3 Tianyu Liu1,2, Tinglin Huang3, Wengong Jin4,5, Tinyi Chu2, Rex4 Ying3, Hongyu Zhao1,2*5 1Interdepartmental Program in Computational Biology &6 Bioinformatics, Yale University...
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  10. ...1 Functional genomics analysis of developing zebrafish and human endoderm reveals 1 highly conserved cis-regulatory modules acting during vertebrate organogenesis 2 3 Daniela M. Riley1,†, Randa Elsayed1,†, Mark D. Walsh2,†, Simaran Johal2, Ying Lin3,4, Harry 4 Walton1, Till Bretschneider5, Sascha...
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