Searching journal content for articles similar to Legrand et al. 27 (9): 1589.

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  1. Method: Single-cell methylome landscapes of mouse embryonic stem cells and early embryos analyzed using reduced representation bisulfite sequencing
    • Hongshan Guo,
    • Ping Zhu,
    • Xinglong Wu,
    • Xianlong Li,
    • Lu Wen,
    • and Fuchou Tang
    Genome Res. December 2013 23: 2126-2135; Published in Advance October 31, 2013, doi:10.1101/gr.161679.113
    ...-base resolution DNA methylation analysis based on reduced representation bisulfite sequencing (scRRBS). The technique is highly sensitive and can detect the methylation status of up to 1.5 million CpG sites within the genome of an individual mouse embryonic stem cell (mESC). Moreover, we show that the technique...
  2. Research: Bacterial infection remodels the DNA methylation landscape of human dendritic cells
    • Alain Pacis,
    • Ludovic Tailleux,
    • Alexander M. Morin,
    • John Lambourne,
    • Julia L. MacIsaac,
    • Vania Yotova,
    • Anne Dumaine,
    • Anne Danckaert,
    • Francesca Luca,
    • Jean-Christophe Grenier,
    • Kasper D. Hansen,
    • Brigitte Gicquel,
    • Miao Yu,
    • Athma Pai,
    • Chuan He,
    • Jenny Tung,
    • Tomi Pastinen,
    • Michael S. Kobor,
    • Roger Pique-Regi,
    • Yoav Gilad,
    • and Luis B. Barreiro
    Genome Res. December 2015 25: 1801-1811; Published in Advance September 21, 2015, doi:10.1101/gr.192005.115
    ...monocytic THP-1 cells (Fig. 1A). At 18 h after infection, we obtained paired data on single base-pair resolution DNA methylation levels (using whole- shotgun bisulfite sequencing, i.e., MethylC-seq) and -wide gene expression data (using mRNA sequencing, i.e., mRNA-seq) in noninfected and MTB-infected DCs...
  3. Research: Systematic mapping of occluded genes by cell fusion reveals prevalence and stability of cis-mediated silencing in somatic cells
    • Timothy J. Looney,
    • Li Zhang,
    • Chih-Hsin Chen,
    • Jae Hyun Lee,
    • Sheila Chari,
    • Frank Fuxiang Mao,
    • Mattia Pelizzola,
    • Lu Zhang,
    • Ryan Lister,
    • Samuel W. Baker,
    • Croydon J. Fernandes,
    • Jedidiah Gaetz,
    • Kara M. Foshay,
    • Kayla L. Clift,
    • Zhenyu Zhang,
    • Wei-Qiang Li,
    • Eric J. Vallender,
    • Ulrich Wagner,
    • Jane Yuxia Qin,
    • Katelyn J. Michelini,
    • Branimir Bugarija,
    • Donghyun Park,
    • Emmanuel Aryee,
    • Thomas Stricker,
    • Jie Zhou,
    • Kevin P. White,
    • Bing Ren,
    • Gary P. Schroth,
    • Joseph R. Ecker,
    • Andy Peng Xiang,
    • and Bruce T. Lahn
    Genome Res. February 2014 24: 267-280; Published in Advance December 5, 2013, doi:10.1101/gr.143891.112
    ...of the two samples by RNA-seq. Bisulfite sequencing of representative genes confirmed that demethlylation had taken place in AdC-treated cells, and that this demethylation persisted in the recovered cells (Supplemental Fig. S8). In the untreated 129TF 3 R1A (clone1), a total of 101 129TF genes were found...
  4. Research: Somatic retrotransposition in the developing rhesus macaque brain
    • Victor Billon,
    • Francisco J. Sanchez-Luque,
    • Jay Rasmussen,
    • Gabriela O. Bodea,
    • Daniel J. Gerhardt,
    • Patricia Gerdes,
    • Seth W. Cheetham,
    • Stephanie N. Schauer,
    • Prabha Ajjikuttira,
    • Thomas J. Meyer,
    • Cora E. Layman,
    • Kimberly A. Nevonen,
    • Natasha Jansz,
    • Jose L. Garcia-Perez,
    • Sandra R. Richardson,
    • Adam D. Ewing,
    • Lucia Carbone,
    • and Geoffrey J. Faulkner
    Genome Res. July 2022 32: 1298-1314; Published in Advance June 21, 2022, doi:10.1101/gr.276451.121
    ...nearly completely methylated (Fig. 3A). We confirmed these results with locus-specific bisulfite sequencing (Fig. 3B,C) and concluded L1RSPRDM4 did not escape methylation in adult tissues.View larger version: In this window In a new window Figure 3. Regulation and embryonic expression of the PRDM4 locus...
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  5. Research: The landscape of genomic imprinting across diverse adult human tissues
    • Yael Baran,
    • Meena Subramaniam,
    • Anne Biton,
    • Taru Tukiainen,
    • Emily K. Tsang,
    • Manuel A. Rivas,
    • Matti Pirinen,
    • Maria Gutierrez-Arcelus,
    • Kevin S. Smith,
    • Kim R. Kukurba,
    • Rui Zhang,
    • Celeste Eng,
    • Dara G. Torgerson,
    • Cydney Urbanek,
    • the GTEx Consortium,
    • Jin Billy Li,
    • Jose R. Rodriguez-Santana,
    • Esteban G. Burchard,
    • Max A. Seibold,
    • Daniel G. MacArthur,
    • Stephen B. Montgomery,
    • Noah A. Zaitlen,
    • and Tuuli Lappalainen
    Genome Res. July 2015 25: 927-936; Published in Advance May 7, 2015, doi:10.1101/gr.192278.115
    ...be difficult, highlighting the value of allelic expression analysis from RNA-seq data and future large-scale bisulfite-sequencing studies where allelic methylation can be distinguished (Kuleshov et al. 2014). Discussion Although imprinting was discovered 30 yr ago (McGrath and Solter 1984; Surani et al. 1984...
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