Searching journal content for articles similar to Lamerdin et al. 5 (4): 359.

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  1. ...on chromosome 1q21. Here we report on the identification of 10 additional EDC genes by a powerful subtractive hybridization method using entire YACs (950_e_2 and 986_e_10) to screen a gridded human keratinocyte cDNA library. Localization of the detected cDNA clones has been established on a long...
  2. ...than span the approximately 2-Mb cri-du-chat critical region (CDCCR) of chromosome 5p to directly screen a chromosome 5-specific (CH5SP) fetal brain cDNA library. To compare this library with other sources for new gene discovery, the YACs were hybridized to a normalized infant brain (NIB) cDNA library...
  3. ...cloning events from the same starting mRNA and usually differ for cDNA preparation protocols, such as normalization, subtraction, cloning vector, or size selection. Mixed libraries were prepared by mixing first-strand cDNAs carrying 6-base sequence tags between the oligo-dT primer and the cloning site...
  4. ...Identified ncRNAs or Their Possible Homologs Found in FANTOM2 Clone Set Computational Screening of the Novel ncRNA Candidates The FANTOM2 clone set contains 60,770 cDNA clones selected from >260 normalized, subtracted, and full-length enriched cDNA libraries of C57BL/6J strain of mouse. The 60,770 clones...
  5. ...expression. [The sequence data described in this paper have been submitted to the GenBank data library under the following accession numbers: AC003061 , mouse BAC clone 245c12; AC003065 , human BAC clone h173(E10); AF053368 , mouse Lor2 cDNA; AF084363 , 108-kb contig from mouse BAC 245c12; AF084364 , mouse D...
  6. ..., J.E., Athwal, R.S. Kansara, M.S. Sandhu, A.K. Patanjali, S.R. Weissman, S.M. and Carrano. A.V. 1995 . Chromosomal localization and expressed sequence tag generation of clones from a normalized human adult thymus cDNA library. Genome Res. 5 : 359 – 367 . Lewanda, A.F., Green, E.D. Weissenbach, J...
  7. .... Construction and characterization of a normalized cDNA library. Proc. Natl. Acad. Sci. 91 : 9228 – 9232 . The Huntington's Disease Collaborative Research Group. 1993 . A novel gene containing a trinucleotide repeat that is expanded and unstable on Huntington's disease chromosomes. Cell 72 : 971 – 983...
  8. .... Nguyen, C., , Rocha, D. , Granjean, S. , Baldit, M. , Bernard, K. , Naquet, P. , Jordan. B. Nguyen, C., Rocha, D. Granjean, S. Baldit, M. Bernard, K. Naquet, P. and Jordan. B. 1995 . Differential gene expression in the murine thymus assayed by quantitative hybridization of arrayed cDNA clones. Genomics...
  9. ...the aggressive normalization procedures used to obtain clones from low-abundance transcripts, there is also a risk some of the putative ncRNA clones were derived from genomic contamination. To address this, we undertook an RT-PCR analysis directed at 74 filtered clones selected at random from a cDNA library...
  10. ...completely identified and confirmed in silico and at the cDNA, mRNA level. In FANTOM2, we have found 90 clones from 33 Kinesin Superfamily Protein (KIF) gene loci. The clones were analyzed in reference to sequence state, library of origin, detection methods, and alternative splicing. More than half...
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