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Research: Plant 24-nt reproductive phasiRNAs from intramolecular duplex mRNAs in diverse monocots
- Atul Kakrana,
- Sandra M. Mathioni,
- Kun Huang,
- Reza Hammond,
- Lee Vandivier,
- Parth Patel,
- Siwaret Arikit,
- Olga Shevchenko,
- Alex E. Harkess,
- Bruce Kingham,
- Brian D. Gregory,
- James H. Leebens-Mack,
- and Blake C. Meyers
Genome Res. September 2018 28: 1333-1344; Published in Advance July 12, 2018, doi:10.1101/gr.228163.117
...) (Supplemental Tables S11, S13). Since 22-nt miRNA targeting leads to RDR6 recruitment, unnecessary for dsRNA precursors formed from IRs, and directs initiation of sRNA phasing, the occurrence of IR/foldback transcripts with miRNA target sites was unexpected. We manually examined a subset of IR precursors...
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Research: Global analyses of the dynamics of mammalian microRNA metabolism
- Elena R. Kingston and
- David P. Bartel
Genome Res. November 2019 29: 1777-1790; Published in Advance September 13, 2019, doi:10.1101/gr.251421.119
...-seq (thiol-linked alkylation for the metabolic sequencing of RNA), has been successfully applied to describe the metabolism of Drosophila miRNAs and siRNAs in S2 cells (Reichholf et al. 2019). However, the need to sequence both the labeled and unlabeled RNA can limit the sensitivity of this method, which...

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