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Review: Leveraging the power of long reads for targeted sequencing
- Shruti V. Iyer,
- Sara Goodwin,
- and William Richard McCombie
Genome Res. November 2024 34: 1701-1718; doi:10.1101/gr.279168.124
...application for early long-read sequencing methods. The typical workflow for long-range PCR long-read sequencing is as follows: High molecular weight (HMW) DNA is first isolated from the target tissue. While this DNA generally does not need to be ultra-long, it must be longer than the ROI. Then primers...
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Method: HD-Marker: a highly multiplexed and flexible approach for targeted genotyping of more than 10,000 genes in a single-tube assay
- Jia Lv,
- Wenqian Jiao,
- Haobing Guo,
- Pingping Liu,
- Ruijia Wang,
- Lingling Zhang,
- Qifan Zeng,
- Xiaoli Hu,
- Zhenmin Bao,
- and Shi Wang
Genome Res. December 2018 28: 1919-1930; Published in Advance November 8, 2018, doi:10.1101/gr.235820.118
...standardized commercial arrays (otherwise requiring large investments in custom array fabrication) (Thomson 2014; Jiang et al. 2016). Highly multiplexed PCR-based approaches, for example, microdroplet PCR (Tewhey et al. 2009) and AmpliSeq (Damiati et al. 2016), and sequence capture approaches like hybrid...
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