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  1. ...,” is replaced with a 22-nt spliced leader. This complicates the mapping of TSSs, leading to a lack of available TSS mapping data for these genes. We used growth at low temperature and nuclear isolation to enrich for transcripts still containing outrons, applying a modified SAGE capture procedure and high...
  2. ...ratios of sense and antisense (S-AS) transcripts between cancer and normal libraries. S-AS transcripts were enriched in known cancer genes, while transcript isoforms were enriched in miRNA targeting sites. We found that transcript abundance had a stronger GC-bias in LongSAGE than Tag-seq, such that AT...
  3. ...is supported by other works. One study used SAGE to exhaustively enumerate the populations of mRNAs within colorectal cancer cell lines ( Velculescu et al. 1999 ) and found TPT1 to be among the most abundant mRNAs in those cell lines. A second study found a yeast homolog to TPT1 to be one of the top 20 most...
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