Searching journal content for articles similar to Khambata-Ford et al. 13 (7): 1765.

Displaying results 1-8 of 8
For checked items
  1. Research: A massively parallel 3′ UTR reporter assay reveals relationships between nucleotide content, sequence conservation, and mRNA destabilization
    • Adam J. Litterman,
    • Robin Kageyama,
    • Olivier Le Tonqueze,
    • Wenxue Zhao,
    • John D. Gagnon,
    • Hani Goodarzi,
    • David J. Erle,
    • and K. Mark Ansel
    Genome Res. June 2019 29: 896-906; Published in Advance May 31, 2019, doi:10.1101/gr.242552.118
    ..., FACS-sorting measurement of protein production, and in both plasmid-based luciferase assays and in vitro–transcribed mRNA-based Kikume reporter assays described in the present work.Our results leave unresolved the specific mechanism of regulation of gene expression by differences in 3′ UTR nucleotide...
  2. Methods: Identification of active transcriptional regulatory modules by the functional assay of DNA from nucleosome-free regions
    • Mahesh Yaragatti,
    • Claudio Basilico,
    • and Lisa Dailey
    Genome Res. June 2008 18: 930-938; Published in Advance April 25, 2008, doi:10.1101/gr.073460.107
    .... , Gleason, C. , Dickson, M. , Altman, R.B. , Batzoglou, S. , Myers, R.M. ( 2003 ) Identification of promoter regions in the human by using a retroviral plasmid library-based functional reporter gene assay . Genome Res. 13 : 1765 – 1774 . ↵ Kim, T.H. , Ren, B. ( 2006 ) Genome-wide analysis of protein...
  3. Review: Advancements in prospective single-cell lineage barcoding and their applications in research
    • Xiaoli Zhang,
    • Yirui Huang,
    • Yajing Yang,
    • Qi-En Wang,
    • and Lang Li
    Genome Res. December 2024 34: 2147-2162; Published in Advance November 21, 2024, doi:10.1101/gr.278944.124
    ...the transduction efficiency. These barcodes are integrated into lentivirus plasmids for efficient delivery and expression within target cells (Figs. 1, 3). When designing the DNA sequence, it is crucial to consider a balance—long enough to ensure a diverse library based on the theoretical size of 4N (N = number...
    OPEN ACCESS ARTICLE
  4. ARTICLE: Retroviruses and yeast retrotransposons use overlapping sets of host genes
    • Becky Irwin,
    • Michael Aye,
    • Pierre Baldi,
    • Nadejda Beliakova-Bethell,
    • Henry Cheng,
    • Yimeng Dou,
    • Willy Liou,
    • and Suzanne Sandmeyer
    Genome Res. May 2005 15: 641-654; Published in Advance April 18, 2005, doi:10.1101/gr.3739005
    ...plasmid, and duplicate independent transformant patches were assayed by two independent observers. In this assay, an active Ty3 element tagged downstream of POL3 with a copy of the HIS3 gene ( Aye and Sandmeyer 2003 ) is induced by growth in galactose as a carbon source. Ty3- HIS3 RNA is reverse...
    OPEN ACCESS ARTICLE
  5. PERSPECTIVE: Qualifying the relationship between sequence conservation and molecular function
    • Gregory M. Cooper and
    • Christopher D. Brown
    Genome Res. February 2008 18: 201-205; doi:10.1101/gr.7205808
    ...in the human by using a retroviral plasmid library-based functional reporter gene assay . Genome Res. 13 : 1765 – 1774 . ↵ Kim, T.H. , Barrera, L.O. , Zheng, M. , Qu, C. , Singer, M.A. , Richmond, T.A. , Wu, Y. , Green, R.D. , Ren, B. ( 2005 ) A high-resolution map of active promoters in the human . Nature 436...
  6. ARTICLE: Distribution and intensity of constraint in mammalian genomic sequence
    • Gregory M. Cooper,
    • Eric A. Stone,
    • George Asimenos,
    • Eric D. Green,
    • Serafim Batzoglou,
    • and Arend Sidow
    Genome Res. July 2005 15: 901-913; Published in Advance June 17, 2005, doi:10.1101/gr.3577405
    ...of purifying selection in mammalian s. Methods Overview of the sequence data Sequences corresponding to the genomic region encompassing the CFTR gene were generated from 28 nonhuman mammals. This represents an expanded version (involving more species) of the data described by Thomas et al. ( 2003 ), with all...
  7. Research: A CRISPR knockout screen identifies SETDB1-target retroelement silencing factors in embryonic stem cells
    • Kei Fukuda,
    • Akihiko Okuda,
    • Kosuke Yusa,
    • and Yoichi Shinkai
    Genome Res. June 2018 28: 846-858; Published in Advance May 4, 2018, doi:10.1101/gr.227280.117
    ....View larger version: In this window In a new window Figure 2. Repeat regulation of top 20 genes and HUSH components. (A) Cluster analysis of the indicated RNA-seq libraries based on log2 fold change of repeats at 5 d after gRNA plasmid transfection. Heatmap color intensity represents Pearson's correlation...
  8. GENOME METHODS: Modification of Bacterial Artificial Chromosome Clones Using Cre Recombinase: Introduction of Selectable Markers for Expression in Eukaryotic Cells
    • Su Young Kim,
    • Stephen K. Horrigan,
    • Janine L. Altenhofen,
    • Zarema H. Arbieva,
    • Ronald Hoffman,
    • and Carol A. Westbrook
    Genome Res. April 1, 1998 8: 404-412; doi:10.1101/gr.8.4.404
    ...retrofitted BAC clones into the SW480 human colorectal cancer cell line. Preliminary experiments using plasmid pEGFP-C1 to introduce the reporter gene GFP showed a transfection efficiency of 25%, as determined by FACS analysis. The same conditions were used to transfect 10 μg of DNA from retrofitted BAC clone...
For checked items

Search Results

New Search

Modify Results

Citation format:
Results / page:
Results order:

This search

  • Alert me when new articles matching this search are published
  • Download all citations on this page to my citation manager

Preprint Server



Current Issue

  1. March 2026, 36 (3)
  1. Current Issue
  1. Alert me to new issues of Genome Research