Searching journal content for articles similar to Kelly et al. 10 (4): 558.

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  1. ...model organism several decades ago and now serves as a primary model for developmental biology, there is only limited data on its genetic variation. An establishment of a dense polymorphism map becomes a requirement for effective linkage analysis and cloning approaches in zebrafish. By comparing ESTs...
  2. ...are indicated under Ygua: 1, 00013F_0; 2, 000200F_0; 3, 000250F_0; 4, 000181F_0; and 5, 000149F_0.A genetic linkage map based on SNP markers deduced from EST and BAC end sequences (Tripathi et al. 2009b) had served as guide for the assembly of both the previously published female and male s. The most distal sex...
  3. ...linkage map of the zebrafish . Shown are the positions of 389 markers genotyped on a single mapping panel consisting of 48 haploid zebrafish embryos. The map includes 157 gene and EST markers, 15 STS markers, and 217 SSLP markers. The SSLPs, which are designated as “z” and “gof” markers, are described...
  4. ...function, we have cloned 11 zebrafish anx genes. Four genes ( anx1a, anx2a, anx5 ,and anx11a) were identified by screening a zebrafish cDNA library with a Xenopus anx2 fragment. For these genes, full-length cDNA sequences were used to cluster 212 EST sequences generated by the Zebrafish Genome...
  5. ...cytogenetically are underlined; those known to represent expressed sequences (identified genes and ESTs) are shown in boldface type. View larger version: In this window In a new window Figure 2. Comparative mapping results among chicken, man, and mouse. The order of the loci is according to the linkage map shown...
  6. ...common ancestor of bony vertebrates. Methods Linkage analysis Primer design, PCR assays, and linkage analysis were performed as previously described ( Kelly et al. 2000 ). In some cases, PCR primers were designed by using alignments of ESTs or genes with genomic DNA derived from the zebrafish whole...
  7. ...genes and expressed sequence tags (ESTs) on zebrafish linkage group LG 17 and the positions of their human counterparts on chromosome (Hsa) 14. ( B ) Maps showing 11 genes and ESTs on zebrafish LG 8 and the positions of their human counterparts on the short arm of Hsa 1. In both panels, the entire...
  8. ...Table 1. Summary of Amplicons Screened and Mutations Found Amplicon Selection and Primer Design As no fully assembled zebrafish is available yet, PCR amplicons for genes of interest were designed from cDNA or EST sequences. These sequences were used as input for GENOTRACE ( Berezikov et al. 2002 ; http...
  9. ...,372 cR, with 118 kb/cR. The distribution of ESTs according to linkage groups shows relatively little variation (minimum, 73; maximum, 201). This observation, combined with a relatively uniform size for zebrafish chromosomes, as previously indicated by karyotyping, indicates that there are no especially...
  10. ...A Gene Expression Screen in Zebrafish Embryogenesis Tetsuhiro Kudoh 1 , Michael Tsang 1 , Neil A. Hukriede , Xiongfong Chen 2 , Michael Dedekian , Christopher J. Clarke , Anne Kiang , Stephanie Schultz , Jonathan...
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