Searching journal content for articles similar to Kajiyama et al. 13 (3): 467.

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  1. METHODS: Homogeneous Assays for Single-Nucleotide Polymorphism Typing Using AlphaScreen
    • Lucille Beaudet,
    • Julie Bédard,
    • Billy Breton,
    • Roberto J. Mercuri,
    • and Marcia L. Budarf
    Genome Res. April 1, 2001 11: 600-608; doi:10.1101/gr.1725501
    .... These competing oligos have been found to increase the specificity of hybridization by the probes. AlphaScreen ASH has been developed for seven SNP assays. The optimal hybridization temperature for allele discrimination was determined using a 96-well gradient thermal cycler in which every column was set...
  2. Research: Coding exons function as tissue-specific enhancers of nearby genes
    • Ramon Y. Birnbaum,
    • E. Josephine Clowney,
    • Orly Agamy,
    • Mee J. Kim,
    • Jingjing Zhao,
    • Takayuki Yamanaka,
    • Zachary Pappalardo,
    • Shoa L. Clarke,
    • Aaron M. Wenger,
    • Loan Nguyen,
    • Fiorella Gurrieri,
    • David B. Everman,
    • Charles E. Schwartz,
    • Ohad S. Birk,
    • Gill Bejerano,
    • Stavros Lomvardas,
    • and Nadav Ahituv
    Genome Res. June 2012 22: 1059-1068; Published in Advance March 22, 2012, doi:10.1101/gr.133546.111
    ...enhancer assays, we demonstrate that several of these exonic enhancer (eExons) candidates can function as enhancers of their neighboring genes and that the exonic sequence is necessary for enhancer activity. Using ChIP, 3C, and DNA FISH, we further show that one of these exonic limb enhancers, Dync1i1...
  3. Research: Widespread somatic L1 retrotransposition occurs early during gastrointestinal cancer evolution
    • Adam D. Ewing,
    • Anthony Gacita,
    • Laura D. Wood,
    • Florence Ma,
    • Dongmei Xing,
    • Min-Sik Kim,
    • Srikanth S. Manda,
    • Gabriela Abril,
    • Gavin Pereira,
    • Alvin Makohon-Moore,
    • Leendert H.J. Looijenga,
    • Ad J.M. Gillis,
    • Ralph H. Hruban,
    • Robert A. Anders,
    • Katharine E. Romans,
    • Akhilesh Pandey,
    • Christine A. Iacobuzio-Donahue,
    • Bert Vogelstein,
    • Kenneth W. Kinzler,
    • Haig H. Kazazian, Jr.,
    • and Szilvia Solyom
    Genome Res. October 2015 25: 1536-1545; Published in Advance August 10, 2015, doi:10.1101/gr.196238.115
    ...), and 12.5 ng DNA were used. Primers were 20 pmol/μL and their location is depicted in Figure 2A. Reactions were incubated for 2min at 95°C, followed by 30 cycles of 30 sec at 95°C, 30 sec at 57°C, and 1.5min at 72°C, followed by final extension of 5 min at 72°C on a Bio-Rad T100 Thermal Cycler. In some GI...
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