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  1. ...the new s, we show that gars have the slowest rates of genomic structural and sequence evolution of all vertebrates. In species of the two living gar genera Atractosteus and Lepisosteus, 83.35% of the s remain identical even though they diverged over 100 million years ago. Genome size variation among gars...
  2. ...effort to reproduce the C. elegans reference from an isogenic derivative of N2 (Table 1), which we published in 2019 as VC2010 (Yoshimura et al. 2019). VC2010 reproduced 100.3 Mb of N2 sequences with 99.98% identity but also contained an extra 1.8 Mb of genomic sequence, along with 10 genomic regions we...
  3. ...Diversity Project (HGDP), which have recently been sequenced to high coverage, are valuable genomic resources because of the global diversity they capture and their open data sharing policies. Here, we harmonized a high-quality set of 4094 whole s from 80 populations in the HGDP and 1kGP with data from...
  4. ...editing efficiency, we amplified genomic DNA from puromycin-selected, doubly transfected C1 cells using primers targeting regions surrounding sites recognized by sgRNAs in RE1, -2, or -3 (Supplemental Fig. S49A). PCR products were analyzed by gel electrophoresis, purified, and sequenced (Supplemental Fig...
  5. ...to be informative for various mRNA metabolism pathways across animals.MethodsStrainsAll novel C. elegans strains were made in N2 background animals (VC2010) (Thompson et al. 2013), and a list of strains is available (Supplemental Table S6).Sample collection for short-read RNA sequencing and library preparation...
  6. ...decreases with increasing genomic distance from a loading source (Brandao et al. 2021). Loop extruders with known recruitment mechanisms support this pattern. ChIP-seq enrichment of bacterial condensin spreads out from the parS sequences (Sullivan et al. 2009; Wang et al. 2017, 2018). Similarly, C. elegans...
  7. ..., gene absence/presence, etc. (Computational Pan-Genomics Consortium 2018).Recent works propose the use of pan references to improve genotyping accuracy from short-read sequencing data (Eggertsson et al. 2017; Sibbesen et al. 2018; Hickey et al. 2020; Letcher et al. 2021; Bradbury et al. 2022; Ebler et...
  8. ...specific genomic sequences while interacting with regulatory proteins, RNA polymerase II, and the chromatin environment to direct gene expression programs. The set of expressed TFs and their activity in a cell determines which genes are expressed and to what level, thereby dictating cellular fate...
  9. ...@mail.hzau.edu.cn, limaoteng426@hust.edu.cnAbstractThe recently constructed mutant libraries of diploid crops by the CRISPR-Cas9 system have provided abundant resources for functional genomics and crop breeding. However, because of the complexity, it is a big challenge to accomplish large-scale targeted mutagenesis...
  10. ...opportunities to uncover novel genomic and epigenomic mechanisms implicated in disease. Sequencing reads generated by LRS not only detect single nucleotide variations (SNVs) and structural variations (SVs) but also probe previously uncharacterized repetitive regions and regions with atypical GC content...
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