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  1. ...transcriptional control that is found in other eukaryotes. The sequences of three Leishmania species have been reported to date, L. major Friedlin (Ivens et al. 2005), L. infantum JPCM5, and L. braziliensis M2904 (Peacock et al. 2007). Comparative genomic analysis of these species revealed highly conserved gene...
  2. ...an overall error rate of 0.86 in 10,000 bases for the final gap-free assembly. Physical Map Verification The complete sequence assembly was verified based on restriction digests of genomic DNA with a panel of three restriction enzymes. DNA fragments were resolved on 1% agarose gels in a pulse...
  3. ....e., HAPPY mapping and optical mapping. HAPPY mapping is an established technique that determines the proximity of DNA markers through PCR assays using multiple pools of diluted, randomly broken genomic DNA (Dear and Cook 1989 , 1993 ). This methodology is technically simple and does not produce large clone...
  4. .... Parasitology 114 : 29 – 35 . ↵ Myler P.J. , Audleman L. , deVos T. , Hixson G. , Kiser P. , Lemley C. , Magness C. , Rickel E. , Sisk E. , Sunkin S. , et al. ( 1999 ) Leishmania major Friedlin chromosome 1 has an unusual distribution of protein-coding genes. Proc. Natl. Acad. Sci. 96 : 2902 – 2906 . ↵ Pays E...
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