Searching journal content for articles similar to Itoh et al. 9 (5): 463.

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  1. REVIEW: Automation for Genomics, Part One: Preparation for Sequencing
    • Deirdre Meldrum
    Genome Res. August 1, 2000 10: 1081-1092; doi:10.1101/gr.101400
    ...with the Japan Science and Technology Corporation (JST), ( http://www-alis.tokyo.jst.go.jp/HGS/top.html )], an automated high-throughput plasmid preparation system has been developed to process 40,000 samples in 17.5 hr. The samples can be used for restriction enzyme digests, DNA sequencing, and transcriptional...
  2. LETTER: RIKEN Integrated Sequence Analysis (RISA) System—384-Format Sequencing Pipeline with 384 Multicapillary Sequencer
    • Kazuhiro Shibata,
    • Masayoshi Itoh,
    • Katsunori Aizawa,
    • Sumiharu Nagaoka,
    • Nobuya Sasaki,
    • Piero Carninci,
    • Hideaki Konno,
    • Junichi Akiyama,
    • Katsuo Nishi,
    • Tokuji Kitsunai,
    • Hideo Tashiro,
    • Mari Itoh,
    • Noriko Sumi,
    • Yoshiyuki Ishii,
    • Shin Nakamura,
    • Makoto Hazama,
    • Tsutomu Nishine,
    • Akira Harada,
    • Rintaro Yamamoto,
    • Hiroyuki Matsumoto,
    • Sumito Sakaguchi,
    • Takashi Ikegami,
    • Katsuya Kashiwagi,
    • Syuji Fujiwake,
    • Kouji Inoue,
    • Yoshiyuki Togawa,
    • Masaki Izawa,
    • Eiji Ohara,
    • Masanori Watahiki,
    • Yuko Yoneda,
    • Tomokazu Ishikawa,
    • Kaori Ozawa,
    • Takumi Tanaka,
    • Shuji Matsuura,
    • Jun Kawai,
    • Yasushi Okazaki,
    • Masami Muramatsu,
    • Yorinao Inoue,
    • Akira Kira,
    • and Yoshihide Hayashizaki
    Genome Res. November 1, 2000 10: 1757-1771; doi:10.1101/gr.152600
    ...plasmid preparator which can handle throughput of 40,000 samples in 17.5 h, and a high-throughput RISA thermal cycler which has four 384-well sites. The combination of these technologies allowed us to construct the RISA system consisting of 16 RISA sequencers, which can process 50,000 DNA samples per day...
  3. METHODS: Normalization and Subtraction of Cap-Trapper-Selected cDNAs to Prepare Full-Length cDNA Libraries for Rapid Discovery of New Genes
    • Piero Carninci,
    • Yuko Shibata,
    • Norihito Hayatsu,
    • Yuichi Sugahara,
    • Kazuhiro Shibata,
    • Masayoshi Itoh,
    • Hideaki Konno,
    • Yasushi Okazaki,
    • Masami Muramatsu,
    • and Yoshihide Hayashizaki
    Genome Res. October 1, 2000 10: 1617-1630; doi:10.1101/gr.145100
    ...the beads with 50 m m NaOH/5 m m EDTA; after neutralization, second-strand cDNA was prepared. Cloning was accomplished in a way analogous to that previously described ( Carninci and Hayashizaki 1999 ). Plasmid was then bulk-excised, and 1000–2000 clones per minilibrary were amplified on agarose...
  4. METHODS: Computer-Based Methods for the Mouse Full-Length cDNA Encyclopedia: Real-Time Sequence Clustering for Construction of a Nonredundant cDNA Library
    • Hideaki Konno,
    • Yoshifumi Fukunishi,
    • Kazuhiro Shibata,
    • Masayoshi Itoh,
    • Piero Carninci,
    • Yuichi Sugahara,
    • and Yoshihide Hayashizaki
    Genome Res. February 1, 2001 11: 281-289; Published in Advance January 18, 2001, doi:10.1101/gr.145701
    .... ( 1999 ) Automated filtration-based high-throughput plasmid preparation system. Genome Res. 9 : 463 – 470 . ↵ Khan A. , Wilcox A.S. , Polymeropoulos M.H. , Hopkins J.A. , Stevens T.J. , Robinson M. , Orpana A.K. , Sikela J.M. ( 1992 ) Single pass sequencing and physical and genetic mapping of human brain...
  5. ARTICLE: Targeting a Complex Transcriptome: The Construction of the Mouse Full-Length cDNA Encyclopedia
    • Piero Carninci,
    • Kazunori Waki,
    • Toshiyuki Shiraki,
    • Hideaki Konno,
    • Kazuhiro Shibata,
    • Masayoshi Itoh,
    • Katsunori Aizawa,
    • Takahiro Arakawa,
    • Yoshiyuki Ishii,
    • Daisuke Sasaki,
    • Hidemasa Bono,
    • Shinji Kondo,
    • Yuichi Sugahara,
    • Rintaro Saito,
    • Naoki Osato,
    • Shiro Fukuda,
    • Kenjiro Sato,
    • Akira Watahiki,
    • Tomoko Hirozane-Kishikawa,
    • Mari Nakamura,
    • Yuko Shibata,
    • Ayako Yasunishi,
    • Noriko Kikuchi,
    • Atsushi Yoshiki,
    • Moriaki Kusakabe,
    • Stefano Gustincich,
    • Kirk Beisel,
    • William Pavan,
    • Vassilis Aidinis,
    • Akira Nakagawara,
    • William A. Held,
    • Hiroo Iwata,
    • Tomohiro Kono,
    • Hiromitsu Nakauchi,
    • Paul Lyons,
    • Christine Wells,
    • David A. Hume,
    • Michela Fagiolini,
    • Takao K. Hensch,
    • Michelle Brinkmeier,
    • Sally Camper,
    • Junji Hirota,
    • Peter Mombaerts,
    • Masami Muramatsu,
    • Yasushi Okazaki,
    • Jun Kawai,
    • and Yoshihide Hayashizaki
    Genome Res. June 2003 13: 1273-1289; doi:10.1101/gr.1119703
    .... 2001 ), which allow routine bulk excision of cloned cDNA into plasmids for sequencing, also permit improved cDNA library diversity. This is due to the peculiar preferential cloning of a larger insert size (2.5∼3.0 Kb), which is markedly larger than cDNA libraries prepared with other available vectors...
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