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  1. ...DNAs by prescreening libraries: 207 new mouse brain ESTs. Genomics 24 : 456 – 463 . Durkin, A.S., , Maglott, D.R. , Nierman. W.C. Durkin, A.S., Maglott, D.R. and Nierman. W.C. 1992 . Chromosomal assignment of 38 human brain expressed sequence tags (ESTs) by analyzing fluorescently labeled PCR products from hybrid cell...
  2. ..., we measured copy numbers experimentally using droplet digital PCR (ddPCR) at 23 different genomic regions. For the majority of the validated loci, the copy numbers determined using ddPCR correlated strongly with those predicted by CNVnator and were largely concordant in most individuals (Supplemental...
  3. ..., with no-RT controls to detect results due to genomic contamination. Expression was confirmed in 198 out of 351 candidates (57%) (Supplemental Table S1). Additional RT-PCR experiments, using RNA from several tissues and variable numbers of PCR cycles, suggested that these SEG candidates are expressed...
  4. ...in A are highlighted in red rectangles in B. Yang et al. 1022 Genome Research www..org two assays for CYP3A4 (3A4T and 3A4M) showed the highest similarity as expected; adjacent to CYP3A4 is the subcluster containing CYP1A2, CYP2C8, and CYP2C9; CYP2C19 and a clade comprised of CYP2E1, CYP2B6, andCYP2A6 are at the next...
  5. ...the junctions betweenNras and mCherry in the of clone 4 and confirmed they were perfectly ligated (data not shown). In applying the same strategy to target primary MEF cells, we could not perform clonal selection, so we genotyped pooled cells 3 d after transfection using genomic PCR with primers specific...
  6. ...CGH analysis of OSW confirmed that result and identified one additional two-copy loss. That second two-copy loss region (confirmed by PCR analysis, data not shown; see Supplemental Methods) is small and results in the deletion of the entire SLITRK1 gene, and otherwise only affects three candidate pseudogenes...
  7. ...obtaining additional sequence information using the RACE PCR method. Genomic DNA hybridizations revealed that it is single copy and the clone was used to screen a total RNA Northern blot. Three signals with approximate sizes of 0.6, 0.8, and 1.8 kb were seen in all tissues with varied intensity...
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