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Methods: Recombinatorial Cloning Using Heterologous Lox Sites
- Robert W. Siegel,
- Nileena Velappan,
- Peter Pavlik,
- Leslie Chasteen,
- and Andrew Bradbury
Genome Res. June 2004 14: 1119-1129; doi:10.1101/gr.1821804
...was devised to expedite the transfer of the scFv gene contained within the phagemid display vector to another destination vector devoid of the bacteriophage coat protein-coding sequence ( Fig. 1A ). The correct recombinant was obtained by imposing antibiotic resistance selection for the desired construct...
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ARTICLE: Generation of a high-resolution genetic map and a YAC contig of the Lurcher locus on mouse chromosome 6.
- J Zuo,
- P L De Jager,
- D J Norman,
- and N Heintz
Genome Res. November 1995 5: 381-392; doi:10.1101/gr.5.4.381
...by screening a YAC library and characterizing YAC end sequences for further screening. This effort has resulted in the construction of a YAC contig consisting of 14 YACs and spanning a 3-Mb region. Markers isolated from these YACs were used to further define the Lc locus, resulting in a physical map...

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