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  1. .... Ligation-anchored PCR: A simple amplification technique with single-sided specificity. Proc. Natl Acad. Set 89 : 9823 – 9825 . 7. Hofmann, M.A. , Brian. D.A. Hofmann, M.A. and Brian. D.A. 1991 . A PCR-enhanced method for determining the 5′ end sequence of mRNAs. PCR Methods Applic. 1 : 43 – 45 . 8...
  2. ....L. and Wolff. J.A. 1991 . A novel 3′ extension technique using random primers in RNA-PCR. Nucleic Acids Res. 19 : 3747 . 17.. Hofmann, M.A. , Brian. D. Hofmann, M.A. and Brian. D. 1991 . A PCR-enhanced method for determining the 5′ sequence of mRNAs. PCR Methods Applic. 1 : 43 – 45 . 18.. Belyavsky, A...
  3. ...genes are based on mRNAs, but can be confounded by artefactual or unprocessed clones in cDNA libraries and by restricted spatial or temporal expression. Finally, genomic sequence from other vertebrates enables identification of conserved sequences ( Hardison et al. 1997 ; Dubchak et al. 2000 ; Mayor et...
  4. ...transcripts in genomic sequences ( Bailey et al. 1998 ; Jiang and Jacob 1998 ; Kan et al. 2001 ), and to study aspects of mRNA structure, such as splicing variants ( Hide et al. 2001 ; Modrek et al. 2001 ; Clark and Thanaraj 2002 ; Kan et al. 2002 ; Xie et al. 2002 ; Xu et al. 2002 ; Wang et al. 2003...
  5. ...Res. 18 : 999 – 1005 . 13.. Bauer, D., , Muller, H. , Reich, J. , Riedel, H. , Ahrenkiel, V. , Warthoe, P. , Strauss. M. Bauer, D., Muller, H. Reich, J. Riedel, H. Ahrenkiel, V. Warthoe, P. and Strauss. M. 1993 . Identification of differentially expressed mRNA species by an improved display technique...
  6. ...sulfoxide (Sigma), or PCR enhancer (Invitrogen) was added to the reaction mixture to improve amplification ( Baskaran et al. 1996 ). The thermal cycling parameter was optimized for each amplicon. See Supplemental Table S1 for detailed conditions for each PCR including primer sequences. The amplified...
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