Searching journal content for articles similar to Hoffmann et al. 12 (1): 98.

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  1. ...to interrogate whether or not DNASE1L3 could cut methylated CpGs. To do this, we first identified methylated and unmethylated CpGs. From a downloaded data set comprised of bisulfite sequencing of eight different mouse tissues (bone marrow, thymus, spleen, kidney, heart, liver, large intestines, small intestines...
  2. ...immune system. However, because the standard gene finding algorithms are not suitable for predicting IG genes, they have been semimanually annotated in very few species. We developed the IGDetective algorithm for predicting IG genes and applied it to species with the assembled IG loci. IGDetective...
  3. ...regulator gene SPI1 and its distal binding sites, which HC is able to restore to normal levels.Myeloproliferative neoplasms (MPNs) are a heterogenous group of hematological disorders characterized by uncontrolled proliferation of hematopoietic stem and progenitor cells in the bone marrow and peripheral...
  4. ...patients with a range of chromosomal abnormalities (The Cancer Genome Atlas Research Network 2013), and hematopoietic stem and progenitor cell (HSPC) populations and B cells from normal fetal bone marrow (FBM) (O'Byrne et al. 2019) to generate individual patient-specific subnetworks derived from our SEM...
  5. ...provides insight into the interplay between DNA methylation and transcriptional networks that are involved in hematopoietic differentiation. Methods Enrichment of primary mouse cells Hematopoietic stem cells and common myeloid progenitor cells were harvested from adult female C57BL/6 bone marrow. After...
  6. ...adult cell-type eosinophils (immune cells infiltrating other tissues). However, we also found expression in immune and blood progenitor tissues such as bone marrow, CD34+ cells from cord blood, and fetal liver and spleen. mR-5 was a sole exception, not being expressed in any of the immune tissues. View...
  7. ...in mouse provides a tool to gain further insights into the role of the human Duffy gene FY. RESULTS AND DISCUSSION Sequence Analysis of Dfy and Bone Marrow cDNA Nucleotide analysis of Dfy and bone marrow cDNA indicated that the structure of Dfy consists of two exons and a single intron (Fig. 1 A). Exon 1...
  8. ...isolation and real-time PCR analyses We isolated total RNA from femurs without bone marrow following the protocol supplied by Life Technologies Company using Trizol reagent. Since the BMD phenotype was analyzed in 16-wk-old female mice, only female mice 14 wk of age were used for gene expression studies...
  9. ..., and undergo apoptotic cell death. C57BL/6J-derived bone marrow and spleen cells cause acute GVHD when transferred into lethally irradiated allogenic male 129P3/J recipients ( Korngold and Sprent 1983 ). To understand the transcriptional changes associated with GVHD, we sampled and analyzed peripheral blood...
  10. ..., direct sequencing of uncloned RT–PCR products generated from a panel of tissues (see Methods) identified the type II transcript in spleen and bone marrow (data not shown). However, our data did not address whether type I and type III isoforms were conserved. View this table: In this window In a new...
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